Luận văn thạc sĩ map based gene cloning of arabidopsis thaliana gametophytic mutant

THAI NGUYEN UNIVERSITY

UNIVERSITY OF AGRICULTURE AND FORESTRY

HA THI LIEU

MAP-BASED GENE CLONING OF ARABIDOPSIS THALIANA

GAMETOPHYTIC MUTANT

BACHELOR THESIS

Study mode : Full time

Major : Biotechnology

Faculty : Biotechnology and Food Technology

Batch : 2013 – 2017

Supervisor : Professor Park Soon Ki

Doctor Pham Bang Phuong

Thai Nguyen – 2017

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ABSTRACT

Thai Nguyen University of Agriculture and Forestry

Major Biotechnology

Student

name

Ha Thi Lieu

Student ID DTN1353150045

Thesis title Map-based gene cloning of Arabidopsis thaliana gametophytic mutant.

Supervisor(s)

Professor Park Soon Ki

Doctor Pham Bang Phuong

Abstract

Arabidopsis thaliana has recently became the organism of choice for a

wide range of studies in plant sciences. Map-based cloning is the

method of identifying a mutation gene by looking for linkage to

markers that physical location in Arabidopsis genome. To find mutation

gene, mutant line, named AP -29 – 38 of Landsberg erecta (Ler) was

out – crossed with Columbia (Col) wild type plant. The F generation

was allowed to do self – pollination and seeds were collected.

Individual F are planted and performed phenotype and genotype

analysis. DNA genome was extracted from leaves of F population.

PCR analysis was conducted using 10 Simple Sequence Length

Polymorphisms (SSLPs) markers to define the chromosome 1 is the

region where mutation located. For fine mapping of mutant gene on

chromosome 1, known or self-made markers were used to narrow

genetic interval down. Candidate genes were sequenced to identify the

mutant gene.

Keywords Arabidopsis thaliana, map-based cloning, AP-29-38

Number of

pages

28

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ACKNOWLEDGEMENT

To accomplish this thesis, first and foremost, I would like to express my sincere

gratitude to the Professor Park Soon Ki and Doctor Oh Sung Aeong, who gave me

helpful advice and guidance during the whole period.

I am thankful to Saima Akhter for her contributions and helpful discussions during the

course.

I would like to thank the members of Sexual Plant Reproduction Laboratory for their

help.

I would also like to extend my heartfelt thanks to Doctor Pham Bang Phuong, who

helped me with useful advice during my internship.

I am grateful to the members of the faculty of Biotechnology and Food Technology for

their support.

Finally, I am especially gratitude to my family and my friends for their

encouragement, generous support and understanding during all of this long campaign

Ha Thi Lieu

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