Luận văn thạc sĩ map based cloning of leaf rolled inside (lri) mutation in arabidopsis thaliana

THAI NGUYEN UNIVERSITY

UNIVERSITY OF AGRICULTURE AND FORESTRY

NGUYEN THU HUONG

MAP - BASED CLONING OF LEAF-ROLLED INSIDE (LRI) MUTATION IN

ARABIDOPSIS THALIANA

BACHELOR THESIS

Study Mode: Full time

Major: Biotechnology

Faculty: Biotechnology and Food Technology

Batch: 2012-2016

Thai Nguyen – 2016

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THAI NGUYEN UNIVERSITY

UNIVERSITY OF AGRICULTURE AND FORESTRY

NGUYEN THU HUONG

MAP - BASED CLONING OF LEAF-ROLLED INSIDE (LRI) MUTATION IN

ARABIDOPSIS THALIANA

BACHELOR THESIS

Study Mode: Full time

Major: Biotechnology

Faculty: Biotechnology and Food Technology

Batch: 2012-2016

Supervisors: Prof. Park Soon Ki ______________________

PhD. Pham Bang Phuong _________________

Thai Nguyen – 2016

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ABSTRACT

Thai Nguyen University of Agriculture and Forestry

Major Biotechnology

Student name Nguyen Thu Huong

Student ID DNT1253150010

Thesis title

Map-based cloning of leaf rolled inside (LRI) mutation in

Arabidopsis thaliana

Supervisor (s)

Prof. Park Soon Ki

PhD. Pham Bang Phuong

Map-based cloning is the method of identifying a mutation gene by looking for

linkage to markers that physical location in the genome. Mutant line, named AP-44-1,

to find a mutation gene, mapping population was conducted and phenotypic analyzed

using F2 plants of mapping lines. Genomic DNA was prepared from leaves of 689 F2

plants for analysis. In order to define chromosomal region where mutation located,

PCR-based mapping was performed using SSLP molecular markers. Finally, the

region containing mutant gene was narrowed down to an approximately 163kb.

Candidate genes in an interval of ~163kb were sequenced to identify the mutant gene.

Combined with the results of An et al (2014) suggesting that mutant gene, At2g32460

caused leaf rolled inside phenotype in AP-44-1 mutant line

Key word Map-based cloning, leaf rolled inside, At2g32460, AP-44-1

Number of

pages

30

Date of

Submission 8/2016

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