Luận văn thạc sĩ development of 3d printed microdialysis probe for determining glucose concentration

Bachelor thesis

THAI NGUYEN UNIVERSITY

UNIVERSITY OF AGRICULTURE AND FORESTRY

DUONG VAN HUY

TOPIC TITTLE: DEVELOPMENT OF 3D-PRINTED MICRODIALYSIS

PROBE FOR DETERMINING GLUCOSE CONCENTRATION IN-VITRO

BACHELOR THESIS

Study Mode : Full-time

Major : Environmental Science and Management

Faculty : International Training and Development Center

Batch : 2010-2015

Thai Nguyen, 22/01/2015

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Bachelor thesis

Huy Van Duong – K42 AEP

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DOCUMENTATION PAGE WITH ABSTRACT

Thai Nguyen University of Agriculture and Forestry

Degree Program Bachelor of Environmental Science and Management

Student name Huy Van Duong

Student ID DTN 1053110103

Thesis Title

Development of 3D-printed Microdialysis probe for

determining glucose concentration In-vitro

Supervisors

1) Prof. Yuh-Chang Sun, National TsingHua

University, Taiwan

2) Assoc. Prof. Dam Xuan Van

Abstract:

To validate the possibility of using 3D-printed microdialysis probe in-Vitro

experiment, in this study we described a methodology of printing 3D-printed probe by

Miicraft 3D printer and assay for testing its efficiency in comparison with the

commercial probe. Perfusate, of testing process, was 0.9% NaCl (Sodium Chloride)

pumped into the inlet of the probe at different flow rate (0.5µL, 1µL, 1.5µL, and 2µL),

flows past the active area of the dialysis membrane, and flow out the outlet of the

probe. Perfusate, in order of passing by the dialysis membrane, a concentration of

glucose is established across the membrane. It facilitates the diffusion of compounds

of interest from the extracellular space through the membrane and also the perfusion

stream for analysis. After all amount of glucose, set previously about 0.3 mL, pumped

into membrane, we collected the sampled glucose from outlet to then analyze and

determine the glucose concentration by using fluorescence microplate reader. To come

up with final result, the relative recovery was formally calculated by given formula.

The desirable efficiency of 3D-printed microdialysis was expected around 70% to 80%

compared that of commercial probe. There were, however, some restrictions as well as

limitations found during the conduction such as inaccurate dimension printing due to

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the size of device was ineligible etc. As a result, the identical efficiency of 3D-printed

probe was unable to reach the announced targets wherein relative recovery of 3D

printed found approximately at 50% to those of introduced device. In detail, the RRs

were found at flow rate of 0.5µL, 1µL, 1.5µL and 2.0µL were around 23.2%, 15.4%,

13.9% and 11.2% respectively in comparison to 42.2%, 35.3% 26.7% and 21.5% of

the introduced probe. Although the result was under the expectation, these data have

proven that 3D-printed probe possibility could be touched in the near future, opens a

wide application of that device to various fields of study.

Key words microdialysis, in-Vitro, 3D-printed microdialysis, relative

recovery.

Number of page 35

Date of

submission

22/01/2015

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ACKNOWLEDGEMENT

This thesis has been greatly conducted from the support as well as assistance of

many people whom I would sincerely like to give deep thanks and acknowledgements here.

Firstly, I would like to send a great acknowledgement to Thai Nguyen

University of Agriculture and Forestry (TUAF) and Advance Education Program

(AEP) for arranging me an appreciated internship to National Tsing Hua University,

Taiwan. Surely, without their helpfulness my study would not come be true.

Secondly, I am deeply grateful to my supervisor, Prof. Yuh-Chang Sun at

Department of Biomedical Engineering and Environmental Science, National Tsing

Hua University (NTHU), Taiwan for accepting, and authorizing me to conduct such a

valuable and potential research during these 3 months. Without his support, this

achievement of my life would not have come to such successes; also, I would like to

say thanks to Assoc. Prof Dam Xuan Van for his enthusiasm in guiding and correcting

my report writing. Both of them deserve a special recognition for their always highly

competent remarks and suggestions and particular praise for their openness and their

calm and friendly manner that permitted him to convey everything most graciously.

Thirdly, I would gratefully like to thank Cheng-Kuan Su, an enthusiastic guider,

he was the one who has had a very positive influence on me and my orientation from

the beginning by suggesting and assisting me this interesting topic during

implementation of the study. Thanks to his amiable disposition and motivational

strength, I could gain new research experiences and noticeably involve in a variety of

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