Đánh giá tác dụng điều trị hội chứng ruột kích thích của Viên nang Thống tả yếu phương trên thực nghiệm

DAN H GI A TAC DUN G DIE U TR! HOI CHUTNG RUOT KICH THiCH

CUA VIEN NANG THONG TA YEU PHUUNG TREN THUC NGHIEM

NGUYEN MINH HA, NGUYI N C6N G THl/C, NGUYI N THI TUYS'T NGA

Vi^n YHCT Quin dgi

HO ANH SON, Hpc vign Quin y

TOM TAT:

Nghien cdu dddc tien hanh nhbm muc dich danh

gia tac dung dieu tri, Hdi ehdng rudt kich thich cda

vien nang Thdng ti yeu phi/ang tren md hinh thdc

nghiem d chudt nhit tring. Ket qua nghien cdu: vien

nang Thdng ta yeu phddng cd tac dung dieu tri Hdi

ehdng rudt kich thich tren thuc nghiem tuang duang

vdi dang thudc sic. Vien nang Thdng ta yeu phuang

cai thien khi ning tieu hda cCia chudt bi gay Hoi

chUng rudt kich thich lam ting trgng luang ca thd

chudt; lam giam cac dau hieu benh ly, thay ddi tinh

chat phan (khdng cdn dang phan long va tao). Ham

lugng nudc trong phan trd ve binh thudng (phan tao

48,9%; phan long 50,2%). Ham lugng serotonin trd ve

vdi mdc binh thudng so vdi trudc khi gay md hinh

(phan tio: 3,36ng/ml; phan long: 4,87ng/ml). Chudt

diiu chinh dugc cac rdi loan ve hanh vi, ting thdi gian

kham phi vung trung tam, ting tiep xuc vdi ddng loai

va cic da'u hieu stress giam dan va trd ve trang thai

binh thudng.

Tu" khda: hgi chung mgt kich thich, md hinh,

serotonin, tinh chat phin, roi logn hinh vi, thong ta yeu

phwang.

Viet tit: Hgi chimg mgt kich thich (HCRKT), Y hgc

c6 tmyen (YHCT), Thong ti yeu phuong (TTYP). . • -.

SUMARRY:

ASSESSING EFFECTIVENESS OF THONG TA YEU

PHUONG CAPSULE ON EXPERIMENTAL MODEL OF

IRRITABLE BOWEL SYNDROME

Our study earned out to evaluate effectiveness of

Thong ta yeu phuong capsule on experimental model

of initable bowel syndrome (IBS). Result shown that:

Thong ta yeu phuong capsule had effect which

treatment IBS as well as Thong ta yeu phuong

extract. Thong ta yeu phuong capsule improved

absorb of food, reduced sign of sick and disorder in

feces (dianrhoea or constipation). Water rate

increased in constipation feces (48.9%) and reduced

in dianhea feces (50.2%). Serotonin plasma came

back nomal level the same as before causing IBS on

mice (constipate 1.36 ng/ml and diarrhoea

4.87ng/ml). Mice controlled behaviour disorder,

stayed in center longer than outside, continued to

contact with other and reduced slowly sign of stress

characters.

Keywords: Thong ta yeu phuong , experimental

model, irritable bowel syndrome • x -

DAT VA'N Di

Hdi chifng rudt kich thich (HCRKT) la mdt hdi

chimg thirdng gap cua diTdng tieu hda bao gdm mdt

nhdm cae trieu chimg nhil: dau bung, chifdng bung.

rdi loan dai tien ... HCRKT khdng de dpa den si/sdng

nhyng hay tai phat do vay anh hirdng nhifiu den chat

lupng cuoc sdng cua b?nh nhan. 6 Viet Nam, theo Ha

van Ngac sd benh nhan tieu hoa tai vien 108 co

24,1%, benh nhan mac HCRKT [1]. Theo Lai Ngoc Thi

phat hien ty le nay la 17,3% [5]. Ty le mac HCRKT 6

nCir gidi nhieu hdn nam gidi, dp tudi trung binh la 40.

Y hoc ed truyen Viet Nam cung da img dung mot

sd bai thudc de dieu trj HCRKT nhiT "Binh vi tan",

"Vien nang He mo" va bai thudc "Tdthan /joan" trong

dieu trj HCRKT the Idng [2],[3],[4]. Bai thudc cd

phiTdng "Thdng ta yeu phuang" (TTYP)da dUpc sir

dyng tir lau b Trung Qudc cung nhir b Viet Nam dl

chiJa chimg can uat ty hu, mot chirng benh ed nhOmg

diem tUdng ddng vdi HCRKT [9]. La bai thudc YHCT

do dd TTYP thirdng diing b dang thudc sic, nghien

ciru chuyen dang thudc thanh vien nang giup ngi/di

benh ed the silr dung d l dang va thuan ldi hdn. Oe

gdp phan nghien eiifu tac dung eCia vien nang so sanh

vdi dang thudc sac chimg tdi tien hanh nghien cidi de

tai nay nham cac muc tieu: danh gia tic dung dieu tri

HCRKT cda vien nang Thdng ta yeu phiTdng" fren

md hinh thuc nghiem.

DOI TUONG VA PHUONG PHAP NGHieN CCfU

Doi tiTdng

Chupt nhit trlng Mus musculus chimg Swiss ca hai

gidi, cd trpng lupng 18-22g tgi thdi dilm nghien cuu, do

Vipn Ve sinh Djeh te Trung uang eung clp. Chupt dupe

nudi trong dilu kipn nhiet dd phdng 24-26°, chieu sSng

theo chu ky 10/14 (bat den liic 8:00 h; tit liic 18:00 h).

Dpng vgt duac cung clp dly dii nudc va thirc Sn,

ehudng trgi hap vp sinh.

Phi/dng phap

Chupt nhit duac chia ngiu nghien thanh 4 Id, m5i 16

20 con.

- Ld 1 (ddi chirng): Khdng gay HCRKT.

- Ld 2 : Gay HCRKT va udng nudc mudi sinh ly

(NMSL).

- Ld 3: Gay HCRKT va udng thudc sic bai Thong ta

ylu phuang lieu 25g duac lipu khd/kg/ngay x 10 ngdy

tuang duang (0,5ml/con cao Idng 1:1).

- Ld 4: Gay HCRKT va udng vien nang Thdng ta yeu

phuang (dgng bao chi hien dgi) lilu 864mg/kg/ngay x

10 ngay.

Chupt duac gay HCRKT bing each nhdt cho vdo

dng nhya hinh try (dudng kinh 2,5cm) va gin vao h$

thdng gia dO, ngam trong nude d nhi§t dp 22,1° C, ngjp

sau 8cm, li§n tyc Igid/ngayxlO ngay.

cac ehi tidu danh gia:

1) Trpng lupng chudt tgi 2 thdi dilm trude va sau

ngam nudc 10 ngay.

18 Y HOC THVC HAN H (741) - S6 11/2010