Xây dựng quy trình chiết xuất và phương pháp định lượng stigmasterol trong thân rễ cây dáy (Alocasia odora (Roxb.) C. Koch) bằng sắc ký lỏng hiệu năng cao

Nghien CLFU - Ky thuat

Xay dyng quy trinh chiet xuat va phuong

phap dmh lu'O'ng stigmasterol trong than re

cay ray {Alocasia odora (Roxb.) C. Koch) > r

bang sac l^y long hieu nang cao

Dat van de

Cay ray {Alocasia odora (Roxb,) C. Koch)

hay cdn gpi la mdn bac ha, ray bac ha, bae ha;

ddi khi cdn gpi la ray dai, da vu. Than rd ray

dugc diing lam thudc trong pham vi kinh nghiem

nhan dan, chu ydu chira benh ngoai da nhu md

day dan ngira, Id khd chii'a, sin da chay nudc

(niu nudc tim rira), xat vao nai bi la han gay

ngira tiy; nhidu nai diing vdi tae dung giam dau

do veo cd. dau xuang khdp, dieu tri gan nhiem

ma

(1.31

Phytpsterpi la mpt trpng nhirng thanh phin

hda hpc eiia eay ray ed heat tinh sinh hpe va

ham lugng cac. Stigmasterel la mdt trpng

nhii'ng thanh phin ehinh eua phytosterol, ed tae

dung giam lugng cholesterol trong mau, khang

viem, dung trong tdng hgp progesteron, va la

tidn chit eiia vitamin D3 '^"^'.

Hien nay, xu hudng va yeu eau tieu chuan

hda chit lugng duge lieu theo WHO ndi ehung

cung nhu d Viet Nam ndi rieng la phai xay dung

dugc quy trinh xac dinh ham lugng boat ehit

hoac chit dac trung chidm ty le ldn trong duge

lieu '^^^. Vi vay, trong bai bao nay, ehiing tdi gidi

thieu kdt qua nghien eiru phuang phap dinh

lugng stigmasterol trong eay ray bing sie ky

long hieu nang cao (HPLC).

ThLPC nghiem nghien CLPU

XLP ly mau nghien CLPU

Than re ray {Alocasia odora (Roxb.) thu hai

vae thang 12 nam 2009, gpt bd vd, rira saeh

bing nude, thai tii'ng lat mdng 2-5mm, siy am

can, dat trong tu siy ddn khd, nghidn min, thu

duae bdt ray, bap quan trpng binh nhua kin.

Tran Hfl-u Dung', Trirong Ngoc Tuyen^

'Dgi hgc Y Dirge Hue,

^Dgi hgc Y Dirge TP. Ho Chi Minh

Chuin stigmasterel 95% cua Sigma-aldrich

(NP: 027K5302), ban Silica gel F2S4 (Merck),

may HPLC SHIMADZU LC-20A series 2000-

PAD detector, cot Supeleo C8 va C18 (150 x

4,6mm, 5pm) va eae dung mdi hoa ehit tinh

khidt (PA va HPLC),

Khao sat he dung moi tridn khai sic ki ldp

mdng dinh tinh stigmasterol

Dung djeh thir: bdt ray khuiy tii' trong 2 gid

vdi CHCIa/MeOH = 2:1 (v/v). Ipe qua gidy Ipc,

Djch chidt dugc thiiy phan bing HCI IM/MeOH

trcng 45 phut d 80°C, sau dd trung hda bing

KOH 20%/MeOH ddn pH > 7, chidt trong ether

diu boa va loai nudc bing Na2S04 khan, cd

dieh ether thu duge ein. Hda tan cin trpng 1ml

CHCI3 dd chim sie ky.

Dung djch ehuin: can 11.4mg stigmasterel

hda tan trong 1ml CHCI3

Thdn khai tren ban Silica gel F254 vdi cac he

dung mdi khae nhau: n-hexan/ethyl aeetat (9:1).

n-hexan/ethyl acetat (8:2), n-hexan/ethyl aeetat

(7:3), CHCIs/MeOH (98:2) va CHCI3/CH3COCH3

(8:2) dd chpn he pha dpng thieh hgp nhit '^',

Phat hien peak pbytesterol bing thude thir

Liebermann-Burchard.

Xay dirng quy trinh chidt stigmasterol

toan phin bing phu'ang phap chidt Soxhlet

Can 5g bpt ray, ngam lanh 12 gid vdi cae he

dung mdi ehidt CHCIj/MeOH (1:1). (2:1) va CHCI3

100%, chidt Soxhlet vdi eiing he dung mdi ngam

lanh trong 3 gid, d 65°C de ehpn he dung mdi

thieh hgp nhit eho viec khao sat thdi gian ehidt

kiet stigmasterol tai 3, 4 va 5h dd chpn thdi gian

chidt tdi uu '^'. Hieu qua ehidt xuit duge danh gia

TAP CHi DUQC HQC-3/2011 (SO 419 NAM 51) 41