Đánh giá hiệu quả quy trình bảo quản tế bào gốc máu ngoại vi thực hiện tại bệnh viện trung ương quân đội 108

TCNCYH 64 (5) - 2009

fusion gene transcript from chromosome with acute promyelocytic leukemia who have

aberrations in acute leukemia for detection of become negative for PML/RARalpha transcript

minimal residual disease: report of the BIOMED - after consolidation therapy; the Japan adult

1 Concerted Action; Investigation of minimal leukemia study group( JASG) APL 97 study. Blood,

residual disease in acute leukemia. Leukemia 13, 1 July 2007Vol 11 0 NOI; 59 - 66.

1901 - 1928. 8. Wetzler M, Bloomfield C D (2000). Acute

7. Norio Asou, Vuji Kishimoto, Hitoshi Kiyoi et and chronic mylogenous leukemia. Harrisons

al (2007). A randomized study with or without Principles of Internal Medicine, 15th ED,

intensified maintenance chemotherapy in patients 584 - 594.

Summary

TREATMENT OF ACUTE PROMYELOCYTIC LEUKEMIA

WITH PML/RARA TRANSCRIPS FUSION

Acute Promyelocytic Leukemia with PML/RARa has good response to ATRA. Objectives: (1) To

determine the efficacy of ATRA and daunorubicin in patients with AML - M3 and PML/RARa. Methods:

33 patients diagnosed AML - M3 by FAB criteria were studied. PCR for PML/RARa fusion transcript was

done before the induction therapy by "ATRA and daunorubicin" protocol and the complete remission was

estimated. Results and conclusions: 1. The median of hospital stay was 19 days, the rate of the patients

have leukopenia with WBC < 1 G/l was: 40%, 2. The complications of the using ATRA include lung

infiltration and increasing pressure of intracranial. 3. Rate of complete remission was 84%, partial

complete remission was 1 6%.

Key words: AML - M3, ATRA, PML/RARa

DANH GIA HIEU QUA QUY TRINH BAO QUAN TE BAO GOC MAU

NGO/\l VI THL/C HIEN TAI BENH VIEN TRUNG U^ONG QUAN DOI 108

Nguyin Thi Thu Ha \ Ly Tuan Khai\ Nguydn Thanh Binh\ TrUOng Thi Minh Nguyet^

Nguydn Duy Hai \ Pham Thj Thu HUdng', Nguyen Xuan Thanh', Do Doan Ldi^

'Benh vien Trung ttang Quin dgi 108; -Trtfdng Dai hge Y Fla ngi

Ddng lanh bao quan khdi ti bio goc miu ngoai vi (PBSC) d nhiet do thi'p (- 196°C) la mgt qui trinh ky

thuit dn thiet trong ghep PBSC. Muc tieu: dng dung qui tnnh bao quin ddng lanh khdi PBSC va dinh gii

hieu qua thdng qua sd lugng vi chai ktang ciia PBSC sau bio quan. Bo'i tugng va phuang phap nghien cdu:

mau PBSC ciia 4 benh nhan ghep PBSC tu than tai benh vien Trung UOng Quan ddi 108 ditgc sd dung trong

nghien cdu.PBSC dugc bio quan theo qui trinh eiia Trung tam ghep tiiy Qud'c gia Hungary. Chat baa quin

ddng lanh la dimethylsulfoxide (DMSO) phd'i hgp vdi hydroxyethylstarch (HES). Ddng lanh tren he thd'ng

thiet bj ha nhiet do cd kiem soil da dugc lap tnnh, luii trd d nhldt do - 196°C trong nita Idng eho den khi sd

dung. Kit qua: ty IS te bio sdng, sd lUdng te bio CD34(+) vi chdc nang cua PBSC dugc duy tn tdt trong thdi

gian bao quin. Thdi gian phuc hoi Heu ciu vi bach ciu hat trung tinh trung binh la 12,25 ± 1,7 ngiy va

11,0 ± 1,0 ngiy. Kit luan: quy trinh ddng lanh bao quan PBSC da duac thuc hien an toin vi cd hieu qua cao.

Td khoa: bao quan dong lanh, te bao goc mau ngoai vi, chat bao quan dong lanh (DMSO, HES)

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