Nghiên cứu tính công hiệu vắc xin viêm não Nhật Bản bất hoạt từ não chuột chủng Beijing - 1 do Việt Nam sản xuất

TCNCYH 71 (dJ - 2010

quality of purified antibodies. Methods: Swiss mice were immunized with purified rabies virus (CVS

strain), immunized mcuse serum were purified by protein G sepharose, the sensitivity and specificity of

experimentally produced antibodies were evaluated and compaired with those of Labvision company by

ELISA and immune electronic microscopy methods. Results and conclusions: the titer of experimentally

purified antibodies met 79.9 ± 0.8 lU/ml; the sensitivity and specificity of experimental antibodies were

equal to those of Labvision company.

Keywords: rabies virus, antibody and purification

NGHIEN CL/U TINH CONG HIEU VACXIN VIEM NAO NHAT BAN

BAT HOAT TLf NAO CHUO T CHUNG BEIJING - 1

DO VIET NAM SAN XUAT

Do Thi Diep Lan

Vien Kiem djnh Qud'c gia Vacxin va Sinh phim y te

Kiim tra cdng hieu li mdt tieu chi rat quan trong di danh gii chi't Idang cda vacxin. Muc tieu: Dinh gia

tinh cdng hieu cua vicxin viem nio Nhit Ban td nao chuot chung Beijing - 1 trong phdng thi nghiem do Viet

Nam san xuat. Boi ttfgng va pbuang phap nghien ctfu: Kiim tra edng hieu vacxin viem nio Nhit Ban bit

hoat td nio chudt chung Beijing - I san xuit tai cdng ty vacxin va sinh phim sd 1 so sinh vdi vicxin miu

chuan Nhit Bin EJP034A. Kit qua: hieu gii khing the trung hoi cua 6 loat vacxin thd nghiem diu Idn han

miu chuan td 0,04 - 0,2 log. Vacxin cd dip dng khang thi tdang ddOng vdi vicxin cua Biken - Nhat Ban. Kit

luan: 6 loat vacxin thd nghiem diu dat yeu ciu vi cdng hieu theo tieu chuin cua WHO vi Viet Nam.

Tii khoa: cong hieu, viem nao Nhat Ban, chung Beijing - 1

LDATVAND E

Benh viem nao Nhat Bin (VNNB) hien dang

la md'i quan tam ldn cua nganh y te el c nflde

chau A vi mde dp nguy hiem cua nd. Benh Iflu

hAnh d chau A va ta't e l cac nflde Ddng Nam A,

vdi khoing 50.000 trfldng hpp mdi nam, trong dd

10.000 ea tfl vong, 1 5.000 ea mang di chflng than

kinh sudt ddi [5].

G i n day, vdi sfl thay ddi eua cac ehung virut

nhfl VNNB genotyp 1 dflpe phat hien d Nhat Ban,

Han Qud'c va Viet Nam... bao dpng cho chdng ta

c i n dfl phdng benh tich efle hpn bang cac loai

vie xin VNNB ed dien dap dng khang the rpng

vdi nhilu ehung, lflu hanh d nhilu vung khae

nhau.Theo nghien eflu cua nhieu tac gi l cho thay

chung Beijing - 1 dflde cci la chung dai dien nhit,

cd the bao ve cheo vdi nhilu chung VNNB lflu

hanh d nhilu dja phflpng va kh i nang mien djch

cao hon ehung Nakayama.

Hien nay, Cdng ty Vacxin va Sinh pham sd 1

(VABIOTECH) da sin xua't vacxin VNNB d el 2

ehung Nakayama va Beijing - T de di p dng nhu

c l u thi trfldng trong va ngoai nflde. Kiem tra cdng

hieu la mpt tieu chf rat quan trpng de dinh gia

chat Ifldng eua vacxin.

Muc tieu: Danh gia tinh cong hieu cua vac

xin viem nao Nhat Ban ttf nao chugt chung

Beijing - 1 do Viet Nam san xuat.

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