Nghiên cứu sự đa hình protein bền nhiệt trong dịch màng bụng và giới hạn phát hiện định lượng protein nhằm thay thế cho xét nghiệm định tính Rivalta

NGHIEN CAU Sir DA HINH PROTEIN BEN NHIET TRONG DjCH MAN G BUNG

VA GlOri HAN PHAT HIE N DjNH LUUDTNG PROTEIN

NHA M THAY THE CHO X£ T NGHIEM DjNH TIN H RIVALTA

v u XUA N TA0 \ NGUYEN GIA BiNH^ LUONG TH| H6N G VAN '

1. Trung tim Nghiin cdv & Chuyin giao Cdng ngh^ Sinh h^c

• Cdng ty CP (fng tl{ing Khoa ftpc Kp thu^t Vl^tNam

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3. Vi$n Khoa hgc S(f sdng - D^i hgc Thii Nguyen

21g/L pmtein content and Rivalta negative gave the

eiectmphomsis msults that was displayed on 5

stmnds. The left ones got the positive Rivalta results

with 37 and 42 g/L protein amount, they all displayed

on 8 stmnds.

Keywords: Heat-msistant pmteins, peritoneal

fluid, polymorphism. Rivalta, transsudat, exsudat.

MdDA U

Cdc djch duyc lly ra t& mdng phdi, mdng tim,

khoang phuc mgc (mdng byng) hodc djch trdn tu cdc

khdp Idn duyc gpi ehung Id djch chpc dd. Ngudi ra

thudng phdn bi0t thdnh 2 logi djch tidt vd djeh thim.

Djeh thim gdp trong cdc trudng hyp th|n hu, xo gan

ed trdn dieh phiic mgc, suy tim. Djch tidt thudng do

nguydn nhdn nhidm khuin, lao, ung thu [1].

Djeh mdng byng Id logi djch ed duyc do sy tieh tg

cdc chit long ty do trong khoang phiie mgc Dich

mdng byng cd thd Id djch thim hode djeh tidt Dich

mdng byng binh thudng khflng vuyt qud 5ml djch

thdm. Djeh mdng byng thudng ed mdu vdng rom.

Xult hi0n vln dye Id do sy hl0n dt0n eua edc bgch

clu tmng tinh. Ngodi ra trong djeh edn cd edc ehit

bdo taing tinh. Trdn djch mdng byng Id mflt dIu hifiu

Idm sdng phd bidn ed nhidu nguydn nhdn nhu: w

gan (75%), ede b0nh dc tinh (10%), suy tim (5%) vS

cdc nguydn nhdn khdc (10%) [4].

Hi0n nay trong vi0c chin dodn rit nhidu bdnh, dSc

bi0t Id ddi vdi cdc b0nh gdy trdn djch mdng byng nhu

lao, ung thu, xo gan, vidm gan, suy gan, suy tim thl

ede kdt qua phdn tlch hda sinh vd sinh hpc phdn tir

ddi vdi djch mdng byng duyc ede nhd chuydn mdn

rdt quan tdm. Nhung cdc nghidn e&u dd cdng bd cua

cdc nhd khoa hpc trong nudc chu ydu tdp tmng vdo

nghidn cuu d$c didm ldm sdng ciia mflt sd bdnh m^

trong dd cd ddc didm trdn djch mdng byng [2J[3]. M$t

sd nghidn e&u cua ede nhd khoa hpe khdc trdn M

gidi dd bit dIu di sdu tim hidu ddc didm sinh hda,

sinh hpc phdn tu eua djch mdng byng dd cung cap

cdc ddu hi0u eho ehln dodn b0nh nhu nhu dya vdo

dya hdm luyng interferon gamma trong dich mSng

byng dd ehln dodn nguydn nhdn gdy lao ciia cSc

bdnh ly [5]; s& dyng ky thudt difln di 2 ehidu 2-D, tia

laser hd try bdi ddi hip thy ion 4 eye (MALDl-Q￾TOP) kdt hyp khdi phd MS. song khdi phd MS/MS fle

nhdn dgng duyc 5 logi protetin cd sy khdc bi0t trong

djch mdng byng eua cdc nhdm ddi tuyng ed bieu

hi0n b0nh ly khde nhau.

T 6 M TAT:

Cdc (dc giA tiin hAnh nghidn cdu phdn tich djnh

tinh, djnh iwQng vA xAc dinh s y da hinh pmtein bin

nhidt tmng djch mdng bung cua cAc bdnh nhdn lao,

ung thw, xa gan, suy tim...cd trAn dieh Kit quA

nghidn cdu trdn 100 miu dich mAng bijng eho thiy

hAm lw(?ng pmtein tdng sd rit cao, tmng binh Id

33.990 ± 15,707g/L. Tmng dd: 27% miu cho kit qui

xdt nghidm Rivalta Am tinh twang dng vdi hdm luryng

pmtein tmng binh IA 18.185± 6,961g/L; 73% miu cho

kit qui xdt nghiim Rivalta dwang tinh twang dng vdi

hAm lwc?ng pmtein tmng blnh IA 46.159 ± 13.907g/L.

Kit quA didn di pmtein bin nhidt theo phwang phdp

SDS - PAGE cho thiy hi pmtein bin nhidt trong dich

mdng bung rit da dang. Sd IwQng bAng (vach pmtein)

thu du%K dao ddng tw 5 din 8 bAng. Miu dich mAng

bijng cd hAm iwang protein IA 21 g/L vA Am tinh

Rivalta cho kit quA didn di xuit hidn 5 bBng. CAc

miu cdn lpi c6 kit quA Rivalta dwang tinh vdi lup'ng

pmtein IA 37 vA 42 g/L xuit hiin 8 bAng.

Td khda: Pmtein bin nhiit, dich mAng bijng. da

hinh. Rivalta, dich thim, dich tiit.

SUMMARY:

RESEARCHING ON THE POLYMORPHISM OF

HEAT-RESISTANT PROTEINS IN PERITONEAL FLUID

AND DETECTING PROTEIN QUANTITATIVE TEST

THAT WILL BE USED INSTEAD OF RIVALTA

QUALITATIVE TEST

The authors carried out a mseamh on

quantitative, qualitative analysis and determine the

polymorphism of heat-resistant pmteins in peritoneal

fluid of patients who got peritoneal effusion diseases

such as: tubemulosis, cancer, cinhosis, congestive

heart failum. The result gained thmugh mseamh on

100 peritoneal fluid samples mvealed that the total

protein content was very high in companson with the

average of 33.990 ± 15.707 g/L. Of that, 27%

samples gained the negative Rivalta test msult

relatively to the 18.185 + 6.961g/L avemge pmtein

content; 73% of the samples gained positive with the

Rivalta test conespondingly to 46.159 ± 13 907g/L

average protein content The electrophoresis results

that were made by SDS - PAGE way showed the

variety of heat-resistant proteins in pentoneal fluid.

The gained number of stmnd (pmtein line) mnged

fmm 5 to 8 stmnds. The peritoneal fluid sample with

Y HOC THU'C HANH (865) - SO 4^13