Nghiên cứu mức độ methyl hóa promoter rassf 1A ở bệnh nhân ung thư tuyến tiền liệt Việt Nam

Tap chi Cong ngh$ Sinh h<?c 11(4): 639-644, 2013

NGHIEN C U ll MU*C DQ METHYL HOA PROMOTER RASSF1A 6 BENH NHAN UNG

THU TUYEN TIEN LIET VIET NAM

V6 Thj Thuwng L an1’2, Nguyen Thj H ong N hung1, Do&n Thj Hdng V an1, Virong Dieu L inhIJ, Ta Van

Tir3

; Truang Dai hQC Khoa hoc tu nhien2, Dai hoc Quoc Gia, Ha Noi

2Genomics Unit, Phong thi nghiem trong diem cong nghe Enzym-Protein, Truang Dai hoc Khoa hoc tu

nhien2, Dai hoc Quoc Gia, Ha Noi

3Khoa Gidi phau benh, Benh vien K Ha Noi

TOM TAT

Ung thu tuyen tien liet dung thu hai trong so cac loai ung thu va la nguyen nhan gay tu vong thu sau trong

cac loai ung thu a nam giai. Nhieu nghien cuu di truyen ngoai gen (epigenetics) da khang djnh su methyl hoa a

cac dao CpG trong vung promoter gen RASSF1A (Ras association domain fam ily I A) lien quan chat che vai

ung thu tuyen tien liet. Do do, trang thai methyl hoa promoter RASSF1A da duoc xem nhu la dau chuan phan

tir dung de chan doan va tien luang sir hinh thanh va tien trien cua ung thu tuyen tien liet. Trong nghien ciru

nay, chung toi thiet ke moi, toi uu hoa dieu kien MSP (methylation-specific polymerase chain reaction) phan

tich sir methyl hoa promoter RASSF1A a 50 mau benh pham due nen duac sinh thiet tir 30 benh nhan ung thu

tuyen (PCa) va 20 benh nhan tang san tuyen (BHP). Methyl hoa RASSFIA dugc phat hien cr 12/30 (40%) benh

nhan PCa va 2/20 (10%) benh nhan BHP. Ket qua nay gop phan khang djnh dau chuan methyl hoa noi chung

va methyl hoa RASSFIA noi rieng co the ap dung trong dieu kien nuac ta de ho tro chan doan va tien luang cac

benh ung thu.

Tir khoa: Di truyen ngoai gen, methyl hoa DNA, khuech dai dac hieu methyl MSP (methylation-specific

polymerase chain reaction), gen RASSFIA (Ras association domain fam ily I A), ung thu tuyen tien liet.

m c s d Au

Ung thu tuyen tien liet la m ot trong so nhung

loai ung thu dudng nieu sinh due thudng gap nhat d

nam gidi va co moi lien quan mat thiet vdi tuoi

(Cairns, 2007). Ung thu tuyen tien liet la nguyen

nhan thu hai gay tu vong do ung thu d nam gidi

(Cairns, 2007). Day la can benh ung thu thudng gap

nhat d nam gidi tai nhung nude phat trien [Cairns,

2007; M anoharan et al.. 2007). Nam 2005, d My co

khoang 230.000 trudng hop dugc chan doan la mac

ung thu tuyen tien liet, chiem 1/3 trong tong so cac

trudng hop mdi mac ung thu d nam gidi va theo du

doan se co 30.000 ngudi chet do loai ung thu nay

moi nam (M anoharan et al., 2007). Chau A nhin

chung co ty le mac mdi ung thu tuyen tien liet thap.

0 Viet Nam, theo nhung bao cao mdi nhat ciia Benh

vien K, benh nay dung thir 12 trong cac can benh

ung thu thudng gap d nam gidi

(w w w .benhvienk.com tin-tuc/ung-thu-tien-liet￾tuyen).

Tham kham true trang va xet nghiem khang

nguyen dac hieu tuyen tien liet (PSA ) la nhung

phirong phap chan doan ung thu tuyen tien liet pho

bien hien nay. Tuy nhien, tham kham thudng chi

phat hien dugc ung thu tuyen d giai doan muon con

gia tri PSA co the cho ket qua duang tinh gia

(Thom pson, 2004).

Trong ung thu tuyen tien liet, mot so lugng Idn

cac gen gom gen sira chua sai hong DNA, gen ire

che khoi u. gen dieu khien kiem soat chu ki te bao,

gen truyen tin hieu. ... co vai tro quan trong trong

hinh thanh va tien trien cua benh. Su bieu hien bat

thudng cua nhung gen nay lien quan mat thiet den

tinh trang benh ly (A hm ed, 2010). Doi vdi ung thu

tuyen tien liet. methyl hoa cytosine trong dao CpG d

prom oter mot so gen xay ra a nhung giai doan dau

cua qua trinh hinh thanh khoi u va su thay doi nay la

dac hieu (Johns et al.. 2010). N hieu nghien ciru cho

thay tinh kha thi khi su dung dau chuan methyl hoa

nhu mot cong cu de chan doan ung thu tuyen tien liet

(Ahm ed. 2010: C aim s. 2007).

RASSF1 (Ras association domain family I A) la

mot gen ire che khoi u tham gia \a o eon dudng dieu

hoa sir phat trien va chet theo chu trinh eua te bao

(Am in. Banerjee. 2012). Doi vdi ung thu tuyen ti£n

liet. methyl hoa gen RASSFIA dat tv le tir 38 - 53%

(M aruyam a et al.. 2002). Dae biet tv le methyl hoa

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