Luận văn algal cell culture in microfluidic devices and microenvironment

THAI NGUYEN UNIVERSITY

UNIVERSITY OF AGRICULTURE AND FORESTRY

HOANG THI MAI

Topic title:

ALGAL CELL CULTURE IN MICROFLUIDIC DEVICES AND

MICROENVIRONMENT

BACHELOR THESIS

Study Mode : Full-time

Major : Biotechnology

Faculty : Biotechnology and Food Technology

Batch : 2013 – 2017

Thai Nguyen, 12/6/2017

c

THAI NGUYEN UNIVERSITY

UNIVERSITY OF AGRICULTURE AND FORESTRY

HOANG THI MAI

Topic title:

ALGAL CELL CULTURE IN MICROFLUIDIC DEVICES AND

MICROENVIRONMENT

BACHELOR THESIS

Study Mode : Full-time

Major : Biotechnology

Faculty : Biotechnology and Food Technology

Batch : 2013 – 2017

Supervisors : Dr. Panwong Kuntanawat

Dr. Nguyen Xuan Vu

Mr. Phongsakorn Kunhorm

Thai Nguyen, 12/6/2017

c

i

DOCUMENTATION PAGE WITH ABSTRACT

Thai Nguyen University of Agriculture and Forestry

Major Biotechnology

Student name Hoang Thi Mai

Student ID DTN1353150021

Thesis title Algal cell culture in microfluidic devices and

microenvironment

Supervisors 1. Dr. Panwong Kuntanawat

2. Dr. Nguyen Xuan Vu

3. Mr.Phongsakorn Kunhorm

Abstract:

Arthrospira platensis is a filamentous multicellular cyanobacterium that has

two distinct shapes: helical and straight filaments. They have high nutritional

value, chemical composition such as protein, pigments, antioxidant, fatty acids.

Microfluidics devices that were applied in various fields such as biological,

biomedical, biotechnology and chemical analyses. A.platensis was captured in

the microfluidics devices in order to observed activation, fragmentation time,

change color, life cycles. It was performed with total 20 filaments (10 filaments

of C005 str and 10 filaments of Central Lab str) in two different conditioned

medium. The result was based on measure length to comparison growth length,

fragmentation time, growth rate of filament and strain. In the standard

Zarrouk’s medium, length and growth rate of Central Lab str is faster than C005

str, fragmentation time is the same. In the stationary from cell culture:

Fragmentation was expressed with two filaments of C005 str (rate 40%) and

three filaments of Central Lab str (rate 60%). Moreover, the growth rate of

Central Lab str was faster than C005 str. The both strains of standard Zarrouk’s

medium were grew faster than Zarrouk’s stationary from cell culture.

Key words C005 str, Central Lab str, microfluidic devices,

growth length, fragmentation time, growth rate.

Number of pages 38

c

ii

ACKNOWLEDGEMENT

Foremost, I would like to express my deep and sincere gratitude to my

supervisor Dr. Panwong Kuntanawat from the School of Bioresources and

Technology, King Mongkut’s University of Technology Thonburi (KMUTT),

Thailand, for providing me the opportunity to conduct research in his lab and

giving me endless support in the past six months. His insights, wisdoms, advices

and enthusiasm for research have greatly influenced me and made the completion

of my dissertation possible.

I would also like to thank Dr. Nguyen Xuan Vu from the Faculty of

Biotechnology and Food of Thai Nguyen University of Agriculture and Forestry

(TUAF) who used to help, support and give me encouragements during this

thesis implementation. I would also like to extend my heartfelt thanks to my

lectures of Biotechnology and Food Department, TUAF who imparted me a lot

of knowledge through four years of university. The knowledge not only helped

me with my research, but also created a basic and soul foundation for me to start

the job in the future. Further, I would also like to express my sincere gratitude to

Ms. Trinh Thi Chung for providing me the opportunity to develop my skills by

doing an internship abroad.

I sincerely thank to the teachers, the laboratory staffs and students at the

laboratory for their regards and giving me an opportunity to do research in the

laboratory. I would also especially thank Mr. Phongsakorn Kunhorm who always

helped, cared, instructed and taught me during my practicing in Thailand.

Finally, I would like to thank my family and my friends for their love and

support. I could not have done this without you.

Many thank and best regards

Student

Hoang Thi Mai

c