Nghiên cứu định danh tế bào tấm biểu mô giác mạc nuôi cấy

TCNCYH 64 (5) - 2009

NGHIEN CL/U DjNH DANH TE BAO

TAM BIEU MO GIAC MAC NUOI CAY

Nguyen Thj Binh, Nguyen Khang Son, Trinh Sinh Tien, Od Thuy HUOng,

Trjnh Binh, Dao Thj Thuy PhUdng, Nguyen Thj Kim Oanh, Nguydn Thu Lan,

Nguyfin Phu Thien, Phan Thj PhUOng, Hoang Thi Thanh

Trtfdng Dai hgc Y Ha Ngi

Sau khi nudi laa thanh cdng ta'm bleu md td te bao gd'c vung na giac mac, viec dinh danh te bao ciia ta'm

bieu md la rat quan trgng. Muc tieu: djnh danh ti bio ciia ta'm bieu md nudi ca'y td te bio goc vung na giic

mac Ciia tho. Boi tugng va phuang phap nghien cdu: sd dung cic ky thuit hiin vi quang hgc, hien vi dien

td, hoi md, hoa md miin djch de phin tich ca'u triic vi the, sieu vi vi eau tnie hoi hgc eiia ti'm bleu md

nudi ca'y. Kit qua: ta'm bieu md nudi dy cd cau tao cua bieu md lit ting khdng sifng hda, 4 - 5 hing te bao:

Kinh hien vi dien tif xuyen cho tha'y cic te bio bleu md d Idp day bim chit via ming d'i. Tai ca dc te bio

lien ke'l chai che vdi nhau bang cic mgng bao tuang vi the Hen ket. Hoi md mien djch chl ra su cd mat ciia

K3, Kl2 trang cic te bao bleu md nudi dy. Kit luan: Ti'm bleu md nudi ciy ttf te bio gd'c viing na ciia thd

la tam bieu md giic mac thd '

Td khoa: Ta'm bieu md nuoi cay, the lien ke't, K3, Kl 2

LDATVAND E

Ton thifOng be mat giac mac nang ne, dai

dang do bdng hoi chit, bdng nhiet hay trong hpi

chdng Stevens Johnson la nhffng thach thdc Idn

vdi cac bac sy nhan khoa. llien nay, de tai tao be

mat giac mac, ci c nha nhan khoa thifdng sd dpng

cac phuong phap: ghep mang di, ghep ke't mac

ria, ghep gilc - cung mac ria. Tuy nhien nhdng

phtfdng phap nay can min h ghep phi i khi Idn

[1, 2] Vai nam gan dly , vdi viec phat trien cua

cdng nghe te bio gd'c, mpt phtfdng phap dang

difpc nghien cdu va dng dung d nhieu nifdc tren

the gidi, dd II ti i tao be mat giac mac bang ghep

tam bieu md gilc mac nudi ciy . Lln di u tien d

Viet Nam, chting tdi da tie'n hanh nudi ci y thanh

cdng tam bieu md giac mac thd td te bio goc

vLing ria giac mac. Viec dinh danh te bao cua tam

bieu md nudi ci y la khau rat quan trpng de khang

dinh te bao cua ti m bieu md nudi ca'y II nhdng te

bio bieu md cua giac mac. Miic tieu:

Binh danh ti bao cua ta'm biiu mo nuoi ca'y

td tc bao gde vung ria.

II. DOI TUONG vA PHUONG PHAP

NGHIEN CL/U

1. Ddi tUdng nghien cdu: Ti m bieu md gilc

mac thd nudi ciy .

2. Phu'dng phap nghien cdu

2.1. Lay manh mo vung ria giac mac cd kicb

thudc 1x1mm. Nudi ci y trong thdi gian 14-1 8

ngay de tao dddc ti m bieu md kich thifdc 4cm'.

2.2. Dinh danb ti bao cua tim biiu mo nuoi

ca'y bang:

Ky thuat hien vi quang hpc:

Quan sit bd mat: sd dung phuong phap nhupm

giemsa (80 mau).

Quan sat mat cat dpc: sd dpng cac tieu bi n

c i t li t nhupm Hematoxylin - Eosin (HE) (80 mlu).

Ky thuat hien vi dien td quet va xuyen (4 mlu).

Ky thuat hoi md: sd dung phtfdng phap phlt

hien glycogen va chit nhay bang phtfong phap

Mac - manus (20 mau)

Ky thuat hoi md mi§n dich de phat hien