Luận văn antimicrobial activity of escherichia coli using n tio2 pmma composite

THAI NGUYEN UNIVERSITY

UNIVERSITY OF AGRICULTURE AND FORESTRY

LE THUY LINH

ANTIMICROBIAL ACTIVITY OF ESCHERICHIA COLI USING N-TiO2-

PMMA COMPOSITE

BACHELOR THESIS

Study Mode : Full-time

Major: Environmental Science and Management

Faculty: International Programs Office

Batch : 2013-2017

Thai Nguyen, 2017

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Thai Nguyen University of Agriculture and Forestry

Degree Program Bachelor of Environmental Science and Management

Student Name Le Thuy Linh

Student ID DTN1353110171

Thesis Title Antimicrobial activity of E. coli using N-TiO2-PMMA

Composite

Supervisor

Assoc. Prof. Nguyen The Hung

Prof. Yao Tung-Lin

Abstract: Titanium dioxide (TiO2) is a semiconductor and photocatalyst in the

decomposition and disinfection of bacteria. In particular, TiO2 were work under

ultraviolet light and visible light. It also had capable of producing a pair of electron

holes due to exposure to ultraviolet and visible light. From that Titanium dioxide can

be used in combination with nitrogen (Nitrogen doped titanium dioxide) to create a

substance that inhibits the activity and growth of bacteria in which clarify ability to

inactivate, especially bacterial Escherichia Coli. This experiment would use the dip￾coating method with antibacterial by Nitrogen doped titanium dioxide (N-TiO2) and

then saw the disinfection activity of E.coli for 24 hours by N-TiO2 0.5%, 1% and 2%.

During experiment took polymethyl methacrylate (PMMA) pieces dip-coating with N￾TiO2 and put PMMA under visible light with the amount 990 to 1010 lux. In all

experiments process, E.coli concentration was dilution by 104 and 105

CFU/ mL. The

results of the experiment showed that E.coli was affected by N-TiO2 antibacterial. The

number of growth and decomposition bacteria has a distinct gap when using N￾n

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TiO2with different percentages. It would reduce the growth of bacteria and cough

complete disinfection around 24 hours. Antibacterial by N-TiO2 has showed as the

optimum one to directly active on bacterial.

Keywords

Titanium dioxide, Nitrogen doped titanium dioxide,

Escherichia coli, Polymethyl Methacrylate.

Number of Pages 43

Date of Submission 20/09/2017

Supervisor’s signature

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ACKNOWLEDGMENTS

From bottom of my heart, I would like to express my deepest appreciation to all those

who provided me the opportunity to complete this research.

First and foremost, I would like to express my sincere gratitude and deep regards to

my supervisor: Pro. Yao Tung-Ling of National Chung Hsing University, Taichung,

Taiwan, who guided me wholeheartedly when I implemented this research. He is

always willing to give suggestions on scientific problems and personal issues. He is

very supportive and a good listener. Under his instruction with patience, knowledge

and talent, I’m able to fulfill to my thesis.

I also want to express my thanks to Assoc. Prof. Nguyen The Hung, the second

supervisor, for his supervision, encouragement, advice, and guidance in writing this

thesis.

In addition, formal thanks should be offered to the Rector of National Chung Hsing

University for granting my internship acceptance.

I want to thank all of my colleagues in the ENM ( Environmental Nano Material)

Laboratory, Miss. Jing-Hua Tzeng, Mr. Chakkrit Poonpakdee, Miss. Li-Ting Yen, Miss.

Ya-Zhen Huang, Mr. Le Quoc Anh, Miss. Kai-Fen, Mr. Che-Chien, Miss. Than Thi Nhu

Anh. They are very generous and supportive. It was a great pleasure to work with them.

I want to express my sincerest appreciation to my parents, my friends and my team for

their love and support me everytime.

Thai Nguyen, 20thSeptember, 2017

Student

Le Thuy Linh

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