Kết quả thử nghiệm dịch ép củ tỏi (Allium Sativum L.) trong phòng trị bệnh do vi khuẩn Streptococcus spp gây ra trên cá trắm cỏ (Ctenopharyngodon Idellus)

KHOA HOC C6NG NOHl

K f-T Q U A THLT l\IG H I$M D |C H E P CL) T b l

[Allium sativum L .) T R O N G P H O N G T R J B $ N H D O

V I K H u A lV Streptococcus s p p G A Y R A T R E N

C A T R A M C O (Ctenopharyngodon Idellus)

Trin Ngpc Hung1, Truong Thj Thdnh Vinh2

t 6 m t At

K£t qua thu nghidm cho thiy djch ep nguydn chat cua cu toi (Allium sativum L) co tinh khang khuin doi voi

vi khuin Streptococcus spp va giam dan b cac muc pha lo&ng, trong do khang khuin tot d nong do 100% voi

duong kinh vong v6 khuin dat 20,75 mm, khang khuin trung binh d cac muc pha loSng 50%, 25%, 12,5% voi

dudng kinh vdng v6 khuin lin lugt dat 17,13, 12,06, 11,13 mm. Djch 6p cu toi voi li£u lugng 40 ml/kg thuc

Sn c6 kha nang phdng b<jnh tot doi voi cA trim co (Ctenopharyngodon idellus) bj b^nh do vi khuin

Streptococcus spp, ty 1$ sdng dat 70%. Dung li£u lugng 80 ml/kg thuc an c6 the? tri khoi b£nh sau 6 ngay

nudi, ty 1$ sdng dat 43,3%.

Tukhda: Tdi, dudng kinh vdng khang khuin, Streptococcus spp.

i . bAtvanb£

Cu toi (Allium sativum L.) dupe dan gian xem la

mOt loai duoc lidu quy, voi thanh phan khang khuan

chu ydu Id chat alexin (QHjoOS^ co tac dung didt

khuan manh, pho didt khuan rpng voi nhidu loai vi

khuan nhu; StaphyUococus sp., thuong han, ph6

thuong hdn, ly, ta, true khudn, bach hdu, vi khuan

gay thoi rua [6]. Chinh vi vay, da co khd nhidu cdc

nghidn cuu vd su dung toi trong phong tri bdnh cho

cdc doi tuong dong vat thuy san. Di(?n hinh nhu san

phdm KN-04-12, BecaNor 1, BecaNor 2 co tac dung

phong vd trj bdnh dom do d ca do Aeromonas

hydrophyla [1]. San pham VTS1-C, VTS1-T phong trj

bdnh do vi khuan Aeromonas hydrophyla,

Edwardsiella tarda, Hafnia alvei, Vibrio harveyi, V

Alginolyticus 6 cd vd tom [7].

Streptococcus spp la mpt trong nhung tdc nhan

gdy bdnh thuong gap trdn cdc doi tupng cd nu6c

ngpt Theo thong kd trdn thd gioi thidt hai do vi

khu&i ndy gdy ra ldn din 150 tridu USD moi nam

[10]. 6 Vidt Nam, da c6 mot so kdt qua nghidn cuu

tai cdc tinh Tdy Ninh, An Giang, Vlnh Long vd Tiln

Giang cho thdy ty Id cd nhidm bdnh ldn ddn 95 -100%

nhdt la vdo mua kho [10].

Nhdn thdy nhung nguy hilm cua tdc nhdn ndy

gdy ra, chung toi tidn hdnh thu nghidm xdc djnh muc

1 Trudng Cdn bp Quan ly Ndng nghidp vd Phdt trien ndng

thon 1.

2 Khoa Ndng Lam Ngu - Trudng D?i hpc Vinh

do khdng khuan va kha nang phong trj cua djch ep

cu toi doi voi vi khuan Streptococcus spp trdn ca

tram co (Ctenopharyngodon idellus), la mot trong so

nhung doi tupng cd nuoi nuoc ngot pho bidn, lam

tidn dd cho nhung nghidn cuu tidp theo tao ra san

pham ung dung trong thuc tidn san xuat

2. VAT UfU, N0I DUNG VA PHUONG PIMP NGHEN CUU

2.1. Dia didm, thdi gian vd v$t lidu nghidn cuu

- Dia dilm nghidn cuu: Phong Vi sinh Bdnh hoc

thuy san, Khoa Nong Lam Ngu, Trudng Dai hpc

Vinh.

- Thdi gian triln khai nghidn cuu: Tir 1/3/2011

ddn 10/5/2012.

- Vat lidu nghidn cuu: Cu toi (Allium sativum L.).

- Doi tupng nghidn cuu: Vi khuan Streptococcus

spp duoc phan lap trdn cd tram co giai doan cd giong

bj bdnh Id loet (khoi lupng cd 9 -10 g/con)

Phuong phdp phan lap vd djnh danh vi khuan

theo Millar vd Frerichs (1993), Buller (2004). Chung

vi khuan sau khi da phan lap dupe cay giCr d 4°C trdn

mdi thudng thach nghidng (Nutrient Broth agar).

Trudc khi su dung kilm tra tinh thuan thdng qua cdc

ddc didm hinh thdi khuan lac, hinh dang kich thudc

vi khuan, nhuom gram, cac chi tidu sinh hda co ban

(oxidaza, catalaza, tinh di dpng, phan ung

decacboxylaza, kha nang ldn men vd oxi hda dudng

glucoza).

N6NG NGHlfP VA PHAT TRIEN NONG THON - KY 1 - THANG 4/2014 87