Hoạt tính sinh học của hai dãy phức chất Trans-, cis - [PtCl2(safrol)(amin)]

• Nghien CLFU - Ky thuat

Hoat tinh sinh hoc cua hai day phirc chat

Trans -, cis - [PtCl2(safrol)(amin)]

Md' dau

Hoat tinh ire chi su phdt then t i bdo ung thu

d ngudi cua cdc phuc chit cis - diaminplatin(ll)

dd thu hut su quan tdm cua rat nhieu nhd khoa

hpc tren thi gidi '^' ^l Mpt trong cdc phuc ehit

dd dd duge su' dung ldm thudc chua trj ung thu

nhu CIS - [PtCl2(NH3)2] vdi ten duge phim Id cis￾platin bode platinol i"*'.

Tuy nhien viee nghien euu kha nang ue ch i

su phdt triin t i bdo ung thu ngudi cua cdc

phue ehit Pt(ll) chua safrol, mpt olefin ed tfnh

chit hudng thin, duge tdch tu tinh dau xd xj

cdn it dugc ehu y.

Trong bai bdo ndy, ehung toi thong bdo v i

kit qua thir boat tinh sinh hpc cua 2 ddy phuc

chat trans -, cis - [PtCl2(safrol)(amin)] tren hai

ddi tugng la vi sinh vdt kiem djnh vd t i bao ung

thu d ngudi. Safrol ed cdng thuc 3,4-

(OCH20)C6H3CH2-CH=CH2, dugc viet tdt Id Sat.

ThLPC nghiem

Tdng hap va xao djnh ciu true cac phirc

Cdc phirc chit trans - [PtCl2(safrole)(amin)] duge

tdng hgp theo phuang trinh phan irng sau '^^':

safrol^ Q

a a

safrok

\ .a

/ ,\ , /

a

\ . amin

+ KQ

•••• '"'•••'- (TI-^TIO)

amin: NH3 (T1), OC4H8NH (T2), CeHnNHi

(T3), 0-CH3C6H4NH2 (T4),

p- CH3C6H4NH2 (T5), 0-CH30CeH4NH2 (T6),

p-CH30CeH4NH2(T7), CgHsNHz (T8), CgHrN:

(T9), CsHsN (T10).

Cdc phirc chit cis - [PtCl2(safrole)(amin)] duge

tdng hgp theo phuong trinh phan ung sau .

\

,C1

/ I't

^ / Cl \ ClJ

safrol \

.Cl

/ I't / safrol \ CI J

+ KCl

(C1,C2, C3)

amin: CsH.oNH (Cl), CeHsNHz (C2),

CH3C6H4NH2 (C3)

Nguyen Thj Thanh Chi, Tran Thi Da

Truang dgi hoc Suphgm Hd Npi

c i u tao eua cdc phirc chit dd dugc xde djnh

nhd phdn tfch hdm lugng nguyen td, do dp din

dien phdn tu kit hgp vdi ede sd lieu v i phd IR,

Raman, phd hip thu electron, phd ''H-NM R vd

"C-NWIRl^'"

Thu> hoat tinh sinh hoc cua cac phu>c chit

Hoat tinh sinh hpc ciia ede phue ehit dugc

thir tai Phong thu nghiem boat tinh sinh hoc -

Vien Hod hpc cdc hgp chit thien nhien - Vien

Khoa hpe vd Cdng nghe Viet Nam.

Hoat tinh khdng vi sinh vdt kiim djnh cua

tit ea cdc phuc chit nghien euu (tru C3)

dugc thu' tren phiin vi lugng 96 giing theo

phuang phdp hien dai cua Vanden Bergher vd

Vlietlinck (1994). Cdc chung vi sinh vdt kiem

dinh bao gdm:

- Vi khuin Gr (-): Escherichia coli (E. coli);

Pseudomonas aeruginosa (P. aeruginosa).

- Vi khuin Gr (+): Bacillus subtilis (B.

subtilis); Staphylococcus aureus (S.aureus).

- Nim mde: Aspergillus niger {Asp. niger);

Fusarium oxysporum (F. oxysporum).

- Nim men: Candida albicans (C. albicans);

Saccharomyces cerevisiae (S. cerevisiae).

Khdng sinh kiim djnh bao gdm : ampicilin ddi

vdi vi khuin Gr (+), tetracyclin ddi vdi vi khuan

Gr (-), amphoterixin B ddi vdi nim moc vd

nystatin ddi vdi nim men.

Miu cd gid trj ndng dp uc ehi tdi thieu (MIC)

< 50 p.g/ml dugc eoi la co boat tfnh.

Hoat tfnh chdng ung thu bang gdy dpc t i

bdo cua cdc phuc chit (T4, T9, T10, Cl , C2,

C3) duge thii' g dang miu chiit tinh tren cdc

dong t i bdo ung thu ngudi nhu Hep-G2 (ung

thu gan), RD (ung thu mdng tim), rieng C3 cdn

dugc thir tren ddng t i bdo LU (ung thu phdi)

theo phuong phdp dang dugc tien hdnh tai Vien

nghien cuu Ung thu Qudc gia My (NCI).

Mlu cd gid tri IC50 (ndng dp uc ch i 50% ddi

tugng thir) < 5n,g/ml dugc coi Id c6 boat tfnh.

K§t qua va thao luan

Kit qua thu- hoat tfnh khdng vi sinh vdt kiem

djnh cua cdc phue chat dugc chT ra a bang 1.

TAP CHI DUQC HOC - 8/2010 (SO 412 NAM 50) 49