Các hợp chất lignan phân lập từ cây dây thần thông Tinospra Tinospra cordifolia (Willd.) Miers

Nghien CLFU — Ky thuat

r ^

Cac hop chat lignan phan lap tir cay day than

thong Tinospra cordifolia (Willd.) Miers

Nguyen Tien DatS Phan Van KiemS Chau Van IMinh',

Dan Thuy HSng', Nguyin Xuan Cudng', Ninh KhSc Ban^,

Nguyin Thj Quy^, Hoang Thanh Huong', Nguyen Van Loi''

' Vien Hda hoc ede hgp ehdt thien nhien,

Vien Khoa hoc vd Cdng nghe Viet Nam

^Vien Sinh thdi vd Tdi nguyen sinh vat,

Vien Khoa hge vd Cdng nghe Viet Nam

^Khoa Sinh, Dgi hge Khoa hge Tu nhien, DHQG Hd Noi

''Khoa Cdng nghe Hda hoc, Dgi hge Bdch khoa Hd Ngi

Dat van de

Day thin th6ng hay eon gpi Id ri gio -

Tinospora cordiflolia (Willd.) Miers, thupe hg Tiit

de - Menispermaeeae phdn bi rpng d vung

D6ng Duang, mpc hoang tren rung niii vd eung

dugc tring de Idm thuic. Cay co vi rit dang,

tinh hdn, khdng dgc, eo tdc dung ta thuc nhiet,

khLP tich tre, tieu u huyet, tan ung dgc, Igi tieu,

ha nhiet, thdng kinh, Igi tieu hod. Trong dan

gian, cay thudng dung Idm thuic be ding, chQ'a

sit ret mdi phdt hay kinh nien ehu'a viem hpng,

no hai diy tire, dai tien tao bdn, bi kinh, kinh

nguyet khong diu. Cay edn duge dung tri thip

khdp, dai dudng. O An Do, ngudi ta diing day

thin thong ehu'a sit ret co chu ky, diing giai

nhiet do sot; bgt ri vd than ehi thuic bo dudng

diing trj Ia chay vd ly man tfnh. Ddy tdn bgt rii

ham uing dung Idm thuic giai khdt vd kich due.

Dieh Id tuai ed tae dung Igi tiiu dugc diing de trj

benh lau. Thdnh phin hda hge ehu yiu cua cay

Id clerodane-diterpen, alkaloid inosporin,

berberin, giloin, giloinin vd lignan '^"'*l Trong

chuang trinh nghien CLPU sdng Igc, tim kiim cdc

hoat chit sinh hge tu ede cdy thuic dan toe cua

ehiing t6i, djch chiit methanol day thin thong ed

boat tfnh khdng mot s i dong t i bdo ung thu in

vitro nhu t i bdo ung thu eo tii' cung Hela (IC50 =

3,78±0,77 pg/mL), SK0V3 (IC50 = 13,29±0,37

pg/mL), t i bdo ung thu vii KPL4 (IC50 =

13,2±0,59 pg/mL), t i bdo ung thu bieu md than

chuyin dang C0S7 (IC50 = 16,14±1,05pg/mL).

Do do, cay ddy thin thdng duge chgn Id dii

tugng nghien euu de tfm kiim cdc hoat chit

42

sinh hge bing cdch tach ehiit, phdn lap cdc hgp

chit Bdi bdo ndy bude Jiu thdng bdo kit qua

phdn lap vd xdc djnh ciu triic hda hge cua 4

hgp ehit khung lignan (1-4) tu day thin thdng,

trong do hgp chit sinapyl 4-0-jS-D￾apiofuranosyl-(1->6)-0-/3-D-glucopyranosid (2)

Id chat mdi, lin diu tien phdn lap tip thien nhien.

Vat lieu va Phu'O'ng phap nghien cii'u

Phu'O'ng phap tach chilt

- Sic ky Idp mdng (TLC) dugc thuc hien tren

ban mong trdng sin DC-Alufolien 60 F254 vd

RP18 F254 (Merck-Dipc). Cdc vit chit dugc phdt

hien bing den tip ngoai d hai bude sdng 254 vd

368 nm hoac diing thuic thCp Id dung djch

H2SO4 10% phun diu len ban mong rii siy d

nhiet do eao cho din khi hien mdu.

- Sic ky cot (CC) dugc tiin hdnh vdi chit

hdp phu pha thudng (Silica gel 240-430 mesh,

Merck) hoac pha dao (ODS-60-14/63, Fujisilisa￾Nhat Ban).

Cac Phu'O'ng phap phd

- Phi khoi lugng phun mil dien tu (ESI-MS)

dugc do tren mdy AGILENT 1200 LC-MSD Trap

cua Vien hda hge cdc ehit thien nhien, Vien

Khoa hpe vd Cdng nghe Viet Nam.

- Phi cpng hudng tip nhan (NMR) dugc do

tren mdy Bruker AM500 FT-NMR Spectrometer,

Vien H6a hge, Vien Khoa hpe vd Cdng nghe

Viet Nam.

Mlu thye vat

Miu day thin thdng Tinospora cordifolia

(Willd.) Miers (Menispermaeeae) (phin tren mat

dit) dugc thu hdi vdo thdng 10 nam 2008 tai

TAP CHI DUQC HQC - 9/2010 (SO 413 NAM 50)