Tiền xử lý rơm rạ bằng axit axetic bổ sung axit clohydric và đường hóa bằng enzyme cho sản xuất

TAP CHi KHOA HQC & CONV, NGHE CAC TRlfOTVG D^l HOC K\ FHIAT * SO 98 - 2014

TIEN XIJ' LY ROM RA BANG AXIT AXETIC B 6 SUNG AXIT CLOHYDRIC VA

DlTCJNG HOA BANG ENZYME CHO SAN XUAT ETANOL SINH HOC

ACETIC ACID WITH ADDED HYDROCHLORIC ACID PRETREATMENT AND ENZYMATIC

HYDROLYSIS OF RICE STRAW FOR BIOETHANOL PRODUCTION

Nguyen Thi Minh Phirffng, Le Quang Dien, Doan Thai Hoa

Trudng Dgi hoc Bdch khoa Hd Ndi

Din Toa soan 26-10-2012, chdp nhdn ddng 25-12-2013

T OM TA T

Bay bao trinh bay kit qua nghien ciru tiin xw ly ram ra bang axit axetic bo sung axit elohydrie va

dwdng hda bing enzyme cho san xuat etanol sinh hoe. Sw thay ddi eau triic nguyen lieu trong qua trinh

xw ly cung da dwgc nghien eiru bang ky thuat hien vi dien tir quet Da xac dinh duxyc che dd edng nghe

thich hgp thu nhan dwdng khCr tir ram ra- tiin xd' ly bing axit axetic bS sung 2% (vi thi tich) axit

elohydrie, a 100°C trong 90 phut, ti dich xw ly 1.10, ket hgp thuy phan bing enzyme cellulase Celtic®

CTec2 eua hang Novozymes V&I hoat Iwe 301,3 FPU/ml), cho ham Iwgngdwdng khw dat 38-39% so vai

nguyen lieu khd tuyet doi (KTD). O diiu kien xd ly nay eo tdi gin 60% nguyen lieu bi chuyen hda. twang

img gin 90% lignin. gin 60% cac chit vd ea bi hda tan. Ngoai ra. tignin cd anh hwang quyit dmh tdi

hieu qua dwdng hda xenluloza

Td khoa Sinh khoi, etanol sinh hoc, tien xie ly, axetic axit, dudng hda being enzyme

ABSTRACT

This paper presents results of study on rice straw pretreatment by acetic acid with added

hydrochloric acid and enzymatic saccharijication for bioelhanol production The sirucliiral changes of

rice straw after treatment has been studied by scanning electronic micro.fcopy (SEM). The appropriate

technology conditions for obtaining of reducing sugar from straw was determined as pretreatment by

acetic acid -with adding of 2% vol. hydrochloric acid, al 100"C for 90 minutes, liquire ratio 1:10 and

combined enzymatic hydrolysis by Novozymes cellulase Cellic® CTec2 with activity of 301.3 FPU/ ml

given yield of reducing sugar over 38-39% of over dry rice straw In these tretreatment conditions about

60% of Ihe /•OM' material has been conversed, 90% of lignin and about 60% of dissolved inorganic

substances have been degraled. In additon. lignin has a decisive influence in cellulose saccharificalion.

Keywords: Biomass, bioethanol, rice straw, pretreatment, acetic acid, saccharification

1. M O DAD gidi, nhieu nghiln cuu cdng nghe tien xu ly rom

M ., , • J-- ,- V,.. • I' J . •- -7 c ra bdng cac tac nhan khde nhau nhu no hoi co Nuoc ta CO dien tich trong lua dat gan 7,5 •, . ^ , , , j , ,. , .-, .-, ,•-, L • • I ]• u- - i',. ' AC xuc tac, nuoc nong ket hop vol kiem, tien xu y trieu ha va san lugng lua hang nam dat tren 45 i . . ° '•- 11, • • ^~ A A

irilu tdn [ I ], Udc tinh co khodng gan 50 trieu tdn bang vi song... da dugc triln khai vd da dat dugc

rom ra phi tlia, sau thu hoach cjn xu iy. La phi ''^l ''"\"!'^ ' ^}"*' pi"!? ' Tuy "hien, theo danh

phu pham n6ng nghiep chira xenluloza va lignin f : "Shuj" cini cdc cong nghe kha thi van la

.-x - -^u-* - J I. • i. ?-i huong nghien CUU can duoc tiep tuc.

tiem nang. co the tan dung cho san xuat nhieu ° ^ . •• '•H 'y--

sanphamcogidtri. nhu hot gidy. nhien lieu sinh 2. VAT LIEU VA PHfON G PHAP

hoc. hda chat,,,, nhung hien nay mdi chi mot n „ J L, I •- - .. ,•• u- J • J ,• L- L-' Roni ra su dung cho nghien ciru dugc thu phan nho ram ra duac su dung am phan bon, , , - u •• k - ,, . x, - r, ,; . •• u-.A'. -.!•- u " . , , •- - gom o huyen Hoai Due, Ha Noi. Rom ra kho gio chat dot. vat heu phuc vu cone, nana nghiep con ^ u-- u- „ • u-; . - .: •- I • u- A', K- - I- • i- • \.u w- ^^^^'^ nghien bang may nghien biia vd sdng o ai b. dot bo,_gay lang phi va anh huong nghiem ^^^ ^^_ ^^^^ ,. ^ ^^.^ ^^^^ ^

trgng den moi truong. p,^.^ ^oa hgc ca ban cua rani ra duge xac djnh

Nghien ciiu liim ndng va tinh chdt ciia theo cac tilu chudn TAPPl d6i vdi nguyen lieu

rom ra [2] cho thdy, day Id nguon nguyln lieu thuc vat.

phii hop cho san xuat etanol sinh hgc. Tren the