Tạo mô sẹo và phát sinh cơ quan từ lá và căn hành cây trinh nữ hoàng cung (Crinum latifolium L.)

Vu Thi Lan va Dig Tgp chi KHOA HQC & CONG NGHE 92(04): 91 -96

TAO MO SEO VA PHAT SINH CO QUAN TlT LA VA CAN HANH

CAY TRINH NU" HOANG CUNG {Crinum lattfolium L.)

Vfl Thi Lan", Nguyen Thj Tinh

Tru&ng Dgi hoc Khoa bgc - DH Thdi Nguyen

TOM TAT

Trong bii bio niy, chiing tdi Uinh biy mpt sd kit qui lilp theo vl tgo md seo cay Trinh nO hoing

cung (TNHC) va sy phit sinh co quan tryc tiep tir li vi cin hinh cua cay. Mau sau khi khu Uiing

dugc ciy Ifin mdi Uudng MS cd bo sung 20 g/l dudng, 10 g/l thach vi cic chit kich thich sinh trudng

vdi ndng dp khic nhau. Cic kit qui thu dugc nhu sau: I) M6i trudng Ihich hgp cho tgo mo spo tir cii

limdi trudng C1|2(2,0 mg/I NAA + 100 mg/I VTMC) vi Cl,5(l,5 mg/I IDA + 100 mg/I VTMC); 2)

Moi trudng phii hgp cho tgo md seo d li in vivo li C1|9(I,5 mg/1 IBA + 100 mg/I VTMC), cdn d li in

vilro li CI2J (2,0 mg/1 IBA + 100 mg/I VTMC); 3) Mdi Uudng thich hgp nhit cho phit sinh chdi tir

vay hanh li TNj (0,5 mg/I NAA). tir li in vitro li MT,o(1,0 mg/I BAP); 4) Mdi Uudng tii sinh chdi li

IB2 (0,5 mg/I IBA + 0,5 mg/1 BAP).

Tfr khoi: Cdn hdnh. cdy Trinh nir hodng cung, md s^o. Id. phdt sinh ca quan

MO DAU

Trinh nii hoang cung (TNHC) thugc hg Thuy

tien dugc nhfin dan Trung Qudc, An Dp, Viet

Nam... sd dung tu lau nhu mpt vi thudc chffa

xung huyet da, viem loet mung mil, bong gfin,

dip mun nhpt, ap xe Djch TNHC lam thudc

nhd chffa dau tai [I], Hipn nay, TNHC trd

thanh cay dugc lieu quy va dugc su dyng

trong vipc chffa trj mpt sd benh cua phy nu vi

nam gidi nhu: u xa, u nang buong trimg, u xo

tiln liet tuyln, dgc biet la tic dyng chdng ung

thu. Gan dfiy, TNHC dupc sii dyng lim

nguyen lipu de bao che mpt sd dugc pham

tren quy md cdng nghiep nhu: vien nang

Crila, Panacrin, Katana, che tan. ..[4]. Do vay,

nhu ciu vl ngudn nguyen lieu TNHC ngay

cing tang.

De tiep tuc gdp phfin vao viec nhfin gidng cay

Trinh nff hoing cung vdi he so nhan gidng

cao va sgch benh chiing ldi da nghien cuu mgt

sd dieu kipn nudi cay md spo vi tii sinh cfiy

tir md seo cfiy Trinh nir hoang cung. Trong

bii bio nay, chung tdi dua ra m^t sd kel qua

tiep theo ciia nhdm nghien cuu ve tao md seo

vi sy phit sinh ca quan tu Ii vi can hanh cay

Trinh nff hoing cung.

VAT LIEU VA PHUONG PHAP

NGHIEN CLTU

Vat lieu: Cfiy Trinh nff hoang cung (Crinum

latifolium L.) thugc hg Thiiy lien

(Amaryllidaceae) do Vien dugc lieu cung cip.

Tel-0914 504250

PhuoTig phap khd triing mau: Li va can

hanh cay TNHC dugc riia sach byi dat bang

xa phdng loang rdi rua dudi vdi nudc chay.

Mfiu li cfiy la nhung li non hoac binh le duac

khff triing bang cdn 70° trong thdi gian 60s va

HgCl2 0,1% trong khoang 10 phiit. Cin hanh

ciy TNHC dugc khir triing bang con 70°

trong thdi gian 60s vi HgCh 0,1% trong

khoang 15 phiit. Cac mau sau khi khir triing

dugc rua lai bang nudc cat vd triing nhieu lan.

Phirong phap bo tri thi nghiem: Cac thi

nghiem dugc tien hanh tren mdi trudng MS

(Murashige - Skoog) vi bd sung 20 g/l

dudng, 10 g/l thach vi cac chit kich thich

sinh trudng vdi cic ndng dp khac nhau.

Chung tdi tien hanh cac nghien ciiu sau : 1)

Anh hudng cua auxin den kha nang tgo md

spo tu li va vay hinh cay TNHC; 2) Anh

hudng cua chat kich thich sinh trudng den sy

phat sinh hinh thai cfiy TNHC; 3) Anh hudng

cua to hgp IBA va BAP den khi nang nhfin

nhanh chdi ciia cfiy TNHC.

Cac chi tieu theo doi: Ty le mau nhiem, ty Ie

mau chit, ty Ie mfiu sdng, ty le tao mo spo, he

sd nhan chdi, chieu cao chdi, ty le tgo re.

KET QUA VA THAO LUAN

Nghien cun kha nang tao mo seo d can

hanh cay TNHC

Nguyen lieu cho lao md seo li cic viy hinh

dugc bdc ra tu can hanh cfiy TNHC cd kich

thudc 1 x 1 cm dugc cfiy len mdi trudng tao

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