Tách dòng gene CRTI mã hóa cho Phytoene Dehydrogenase từ Pantoea Ananatis

HQI NGH! KHOA HQC cCiA HQI HOA SINH Y DlJQC HA NQI VA CAC TJNH PHIA BAC LAN THUf XVII

TACH DONG GENE CRTI MA HOA CHO PHYTOENE DEHYDROGENASE

TUT PANTOEAANANATIS

Nguyen Thi Hirong*, Nguyen Xuan Vu*, Dirong Van Cu-dng*

TOM TA T

Lycopene la sac td mau dd thudc nhdm

carotenoid. Sac td nay la chat chdng oxi hda hieu

qua va da du'dc chu'ng minh la cd kha nang

chdng lai mdt sd benh ung thu' nhat dmh, nhu'

ung thu' tuyen tien liet. Hien nay, ngudn cung

cap lycopene tu" tu" nhien bang phu'dng phap

chiet xuat tCr mdt sd loai qua nhu' gac, ca chua,

bu'di dd... cd mdt sd yeu diem nhu' phu thudc

thdi vu va khd tien hanh d quy md Idn. Do vay,

can xay di/ng cac ngudn cung cap lycopene nhan

tao. Trong con du'dng tdng hdp lycopene, gene

crti ma hda cho enzyme phytoene

dehydrogenease xiic tac cho phan liYig chuyen

phytoen thanh lycopene. Trong nghien cii\i nay

chiing toi da tach ddng va xac djnh binh tu" gene atl

tu' vi khuan Pantos ananatis. Ket qua thu du'dc

gene crtfciia Pantoea ananatis &Seu dai 1479bp cd

binh hi tu'dng dong 100% vcfi hinh tiT gene atl

phan lap tii Pantoea ananatis C& cdng bd hen ngan

hang gene NCBI ma sd D90087.2. Ket qua ciia

nghien ciJu nay la tien de cho muc tieu tao chiing £

colica kha nang san xuat lycopene.

Tu* khoa: Lycopene, phytoene

dehydrogenease, crtI, carotenoid

SUMMARY

CLONING OF c/tTGENE CODING FOR

PHYTOENE DEHYDROGENASE FROM

Pantoea ananatis

Abstract Lycopene, a red carotenoid

pigment, is an effective antioxidant which has

been shown to have preventive effects against

some cancers, e.g., prostate cancer. Currently,

the commercial lycopene is provided by natural

sources which are facing remarkable

disadvantages such as season dependence and

difficulty in larger scale. It is necessary to

* Khoa Cong ngh? Sinh hoc va Cong ngh? Thvrc phdm,

Trudng Dgi hoc Nong Ldm, Dgi hoc Thdi Nguyen

generate other sources of artificial lycopene

production. In the lycopene synthesis pathway,

phytoene dehydrogenease, encoded by crtI

gene, catalyzes the conversion reaction of

phytoen to lycopene. In this study, we have

cloned and sequenced crtI gene from Pantoea

ananatis. The sequence of the cloned 1479 bp

fragment is 100% matched with the previously

published crtl isolated from Pantoea ananatis

Gene bank number D90087.2. The cloned cfff

will be used for our upcoming studies of E coll

metabolic engineering to produce carotenoid.

Keywords: Lycopene, phytoene

dehydrogenease, crtl, carotenoid

I. OAT VAN oi

Carotenoid la nhdm sdc td tu nhidn dugc

tim thdy trong cac sinh v|it bao gdm thuc vat,

ddng vat, vi khuan, tao, ndm (Das et al,

2007). Carotenoid vdi vai trd la chdt chdng

oxi hda da mang lai Igi ich cho siirc khde con

ngudi nhu ngan ngira hay tri hoSn cac bgrih

man tinh nhu ung thu, xa ciing ddng mach,

due thiiy tinh the va mdt sd bdnh khac

(Campbell et al, 2010). Carotenoid dugc sur

dung lam chat mau thuc pham, chat bd sung

vao thiic an chan nudi va gdn day dugc sit

dimg trong nghanh cdng nghiep san xudt my

phdm va dugc phdm (Schmidt-Dannert,

2002). Theo bao cao cua td chiic BCC

Research, thi trudng carotenoid toan cdu nam

2007 vao khoang 766 trigu USD va d\r doan

tang len 919 trieu USD vao nam 2015.

Lycopene la mdt trong nhihig sac to

chinh trong nhdm carotenoid dugc sii dyng

rgng rai trong cdng nghiep. Sdc td nay cd kha

nang bao vd td bao khdi su oxi hda (Sies and

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