Sử dụng máy CASA trong xét nghiệm tinh dịch và những điểm cần lưu ý khi trả lời kết quả xét nghiệm tinh dịch

sur DONG MAY CASA TRONG XET NGHIEM TINH DjCH VA NHUNG DIEM CAN LOU Y

KHI TRA LQII KET QUA XET NGHIEM TINH DjCH

TRAN OL/C PHAN, PHAN THj HOAN, NGUY§N XUAN TUNG

Bo Mon Y sinh hgc - Di truyen • DHYHN

TOM TAT

Xet nghiem tinh djeh la mdt xet nghiem ca ban di

chan doin vi theo ddi qua trinh dieu trj. Ngay nay cd

nhieu thiet bi cd thS hd trg tot cho viec phin tich tinh

dich, miy CASA li mdt trong cic thiet bj dd. Bio cao

nay chimg tdi de cap den 2 van de sau:

- Cic chi so xet nghiem tinh dich va y nghTa cua

chung.

- NhOmg uu diSm vi han che ciia miy CASA khi

xet nghiem tinh djeh.

Miy CASA hd trg phan tich nhieu chi so tinh djeh

nhanh, chinh xie. Miy die biet cd uu the khi phin

tich do di ddng cCia tinh trimg, phin tich mat do cua

tinh trung. Cd cic chi so mi phan tich bing mit qua

kinh hi6n vi khdng lim dugc nhu do toe do di chuyen

ciia tinh triing, phan loai hinh thii di chuyen cCia tinh

trimg...; cac chi so niy chicd thi phan tich bing miy

nhu miy CASA. NhOng trudng hgp thiSu tinh ning vi

vd tinh thi budc phai phan tich bing tay.

Chat lugng xet nghiem tinh djeh nen dugc chu y

ting cudng. Nen dua them cic chi so nhu do toe do

di chuyin cua tinh trimg, phan loai hinh thai di chuyin

cua tinh triing vio xet nghiem thudng quy. Ve hinh

thii, nen dua chi so tinh triing dau hinh kim vi khdng

cd diu vio xet nghiem thudng quy vi cic loai tinh

triing nay anh hudng nhieu den chat lugng thu tinh

cua tinh trimg.

TU khda: Xet nghiem tinh djeh, may CASA

SUMMARY

Using CASA for semen analysis and some

main point need to pay attention when we give

infertility counseling

For diagnosis and monitoring the results of male

infertility treatment, semen analysis is very important

test. Nowadays, some equipments are useful for

semen analysis, one of them is Computer Assisted

Sperm Analysis (CASA). 2 objectives would be

mentioned in this report:

- Semen indicators and the role of them for

infertility diagnosis.

- Investigate strong and weak point of CASA

machine and find out the good way to use it.

Carried out this study, we found that:

- Using CASA, we could analysis sperm very

quick, accurate..., especially for classify the kind of

sperm motility, measure the speed of sperm, identify

the form of sperm motility and sperm concentrate.

Some indicators like speed of sperm, the form of

sperm motility couldn't be Identified by hand.

- In case serious oligospermia and azospermia,

we must analysis sperm by hand.

Some more indicators we need put in routine test

of semen: speed of sperm, form of sperm motility,

sperm with needle head shape, non head.

Keywords: CASA, semen analysis

OAT VAN OE

Khd cd eon hay "Thieu nang sinh san" (TNSS) la

tinh trang ehi nhung cap vp chdng ed hoat dpng sinh

due binh thudng, khdng diing bat cG bien phap tranh

thai nao ma sau it nha't 12 thang vdn khdng cd thai (6,

8). Theo Baker (1993) (trich ddn theo 6), d cae cap vd

chdng cd TNSS: 1/3 do ngUdi chdng, 1/3 do ngUdi vd

va 1/3 sd cap edn lai co nguyen nhan do ca vd vd

chdng.

Xet nghiem tinh dich la mot xet nghiem cd ban

khdng the thieu dUde de cha'n doan tinh trang TNSS

nam. Nd cung cdn la xet nghiem dung de theo doi

qua trinh dieu trj.

Xet nghiem nay ed nhieu ehi sd chi tiet chUa dUdc

Slf dung, ed cac chi sd ehUa cd cac phUdng phap xac

dinh mdt each chinh xac [3, 5, 7]. Vl vay, cho du da

cd nhieu ky thuat mdi ra ddi, song vdn cd nhieu

trudng hdp viec chan doan nguyen nhan cdn rat khd

khan [8]. May CASA (computer assisted sperm

analysis) Id may phan tfeh tinh dich nhanh, ehfnh xae,

phdn tfeh ehi tiet dupe nhieu ehi sd nhung cung ed

eae han ehe.

Oe cd cai nhin tdng the ve eae ehi sd xet nghiem

tinh djeh, y nghTa eua eae chi sd xet nghiem tinh djeh

vd chat lUdng cua xet nghiem tinh djeh bang may

CASA, bdo eao nay chung tdi de cap den 2 van de

sau:

- Cac chi sd xet nghiem tinh dich va y nghTa eua

chiing.

- NhOng UU diem va han che cua may CASA khi

xet nghiem tinh dich.

DOI TL/pNG VA PHl/ONG PHAP NGHIEN CQU

1. Doi tifdng

Odi tUdng nghien cifu eua chung tdi la nhifng

ngudi nam gidi, trong cac cap TNSS den xet nghiem

tai bd mdn Y sinh hoe - Di truyen trudng dai hoe Y Ha

Npi.'

2. PhUdng phap nghien ciiru

Chiing tdi sif dung phUdng phap nghien ciru md ta

so sanh. Cae mau tinh dich dUde phan ti'ch theo tieu

chuan eua WHO 1992, 1995, 1999 [5, 7, 9].

Oe danh gia chat lUdng eua xet nghiem tinh djeh

bang may CASA, mdi mau dUdc phan tfeh 2 lan bang

mat dudi kinh hien vi va 2 lan bang may CASA.

3. Cac ch? so xet nghiem tinh djeh

3.1. Khao sat dai the

Y HOC THl/C HANH (727) - SO 7/2010 55