Sản xuất kháng thể kháng VI dùng trong kiểm định vắc xin thương hàn VI

SAN XUAT KHANG THE KHANG Vi

DUNG TRONG KIEM DjNH VAC XIN THlAJNG HAN Vi

LE THj HOANG YEN, LlfU ANH THI/,

NGUYEN MANH KHAI, NGUYEN THI. HONG LINH

Vi§n Kiem djnh Quoc gia Vac xin v i Sinh Pham Y te (NICVB).

t 6 m tAt

SSn xuat khSng thS khSng Vi dung trong kiSm

dinh ch£t tuxyng vSc xin thuxyng han Vi IS r£t c in thiSt

nhdm giam chi phi vd chu dong trong cdng vi$c. Muc

ti&u: Dwa ra duxyc quy trinh chuan trong san xuat

kh6ng thS khcing Vi tai Vien Kiem dinh Quoc gia VSc

xin vS Sinh Pham Y t4. Doi tuxyng vS phuxmg phip

nghien c lp u : doi tuxyng nghien clpu IS quy trinh sSn

x u it khSng thS khSng Vi cua My cd cSi tiSn vS giong

thd Newzealand duxyc cung cap bdi khoa dong vSt

thi/c nghiem cua NICVB. Thd duxyc miSn djch vdi

huySn dich chung Citrobacter freundii. Ket quit: Ty I$

thd cho khSng thS khSng Vi dat yeu c&u IS khoSng

86,7%.

Tip khoi: KhSng the khSng Vi, nh$n dang Vi, ham

tuxyng Vi, Citrobacter freundii, thd Newzealand

PRODUCTION OF SERUM ANTI-Vi USED IN

QUALITY CONTROL Vi TYPHOID VACCINE IN

NATIONAL INSTITUTE FOR CONTROL OF VACCINE

AND BIOLOGICAL

SUM MARY

Production of serum anti-Vi used in quality control

Vi typhoid vaccine is necessary to reduce fees and

proactive work. Objective: Establish the standard

procedure in the production of serum anti-Vi in the

National Institute for Control of Vaccine and

Biologicals. Subjects and Methods : the research

object is based on the USA production process of

serum anti-Vi from New Zealand rabbit provided by

Animals Department of NICVB. The rabbits are

immunized by Citrobacter freundii suspension.

Results. Rate of rabbit serum anti-Vi is satisfactory

(approximately 86.7 %).

Keywords: Antibody Vi, Vi identity, Vi

polysaccharide concentration, Citrobacter freundii,

New Zealand rabbit

DAT VAN 0£

Thuang han !£ benh truyen nhiim I3y qua dudng

tieu h6a, cap tinh gay thanh dich, phat tin rim ro, do

vi khuin S. typhi, S. paratyphi A, S. paratyphi B, S.

paratyphi C. O’ Viet Nam, trong “Luat phong, chong

b§nh truyen nhiim ”, benh thuong hdn x ip vao nhom

benh I3y truyen nhom B, lay phdng benh Id chinh

trong dd cd thdng tin, giao due, truyen thdng, gi£m

sat bdnh truyen nhiim , tiem phdng v ie xin ket hop

cSc bidn phdp chuyen mon ky thuat y te trong phdng,

chong benh truyen nhiim [4],

VSc xin thuong han ra ddi da gdp phan giam ty le

m lc vd tu vong do benh thuong han [9], De dam bao

ch it luong v ie xin thuong hdn Vi (san xu it tu chung

S. typhi Ty2), viec kiem tra chat luong vie xin Id cin

thiet vS b it buoc. Mdt trong nhung sinh phim quan

trong dung de kiem dinh v ie xin thuong han Vi la

kh£ng the khdng Vi. Viec mua huyet thanh khang Vi

tu nude ngoai rat d lt khd van chuyen, ddi hdi tuan

thu chat che quy trinh ddy truyin lanh, khdng chu

dong trong cdng tdc kiem dinh tai Vien kiem dinh

quoc gia v ie xin va sinh phim y te.

Chung Citrobacter freundii cd c iu tao KN Vi giong

vdi KN Vi chung S. typhi Ty2. Chinh vi v|y, WHO

khuyen cdo san xu it KT Vi dung cho kiem dinh vie

xin thuong hdn Vi tu chung Citrobacter freundii [10],

[11], Viec san xu it KT Vi tu chung Citrobacter

freundii don gian hon, an toan hon, vi dSy \S chung vi

khuin khdng doc, khi g£y m iin dich tren thd cho kha

ndng tao dap ung m iin dich tdt vd thu duoc KT Vi d

muc kha quan. Hon nua, viec tao KT Vi tu chung

Citrobacter freundii khdng xay ra hien tuong ngung

ket cheo vdi cac khdng nguyen khac ngoai KN Vi cua

chung S. typhi Ty2.

Do vay, chung tdi thuc hien nghien cuu ndy vdi

muc tieu:

- Xay dung quy trinh san x u it huyet thanh khang

Vi tren thd bing chung Citrobacter freundii WR7011

tai Vien Kiem dinh Quoc gia V ic xin va Sinh pham Y

t i [3], [11].

VAT LIEU VA PHU’ONG PHAP NGHIEN Cl>U

1. Vat lieu nghien c lp u

- Chung san xuit: C. freundii WR7011.

- Thd cai Newzealand khoe manh, trong luong 2,0

- 3,0kg/con, cd huyit thanh n in (-) vdi KN Vi.

- Quy trinh san x u it khang th i khang Vi theo

phu’ong phap cua My cd cai tiin.

- Mdi trudng nuoi c iy la TSB (Tryptic Soy Broth),

TSA (Tryptic Soy Agar) va cac hoa chat dung trong

nghien cuu cua Merck, Due.

2. Phu’ang phap

2.1. Tao huyen dich chung

Ly tam huyin dich chung C.freundii W7011 da

duoc nuoi c iy 5 gid trong moi trudng TSB, loai bd

dich noi, thu phin tua Pha loang phin tua trong dem

PBS roi b it hoat bing dung dich formaldehyde 0,5%.

Sau khi b it hoat, d i huyin dich trong tu lanh 2 - 8°C

tu 12 - 18 gid L iy Idp lin g mau trlng sua, loai bd

Idp nude trong ben tren va phin lin g can mau nau d

ben dudi. Su dung dung dich nude muoi sinh ly

0,85% pha loang, de duoc huyin dich cd mat do

quang OD = 2,0 tai bu-dc sdng X=550nm [5],

2.2. Gay mien dich

Thd duoc tiem m iin djch (tiem b lp va tinh mach)

V/ u r w - T H ir r H A N H <922) - SO 6/2014 31