Sản xuất kháng thể kháng dại gắn huỳnh quang

SAN XUAT KHANG THE KHANG DAI GAN HUYNH QUANG

Nguyin Thj Kieu Anh, Nguyin VTnh Dong, Ngo Chau Giang,

Nguyin Tuyet Thu, Nguyen Thj Hong Hanh

Vien ve sinh Djch ti Trung Udng

Khang the khang dai gin huynh quang cd dng dung rdng rai trong chin doan va nghidn cdu virus dai, do

vay vide nghien cdu san xua't de dip dng nhu cau sd dung d trong nddc li hit sdc cin thiet. Muc tieu:

nghien cdu san xua't cdng hap khing the khang dai gin huynh quang (FITC); Lfng dung cdng hap san xuat

thif nghiem de chuin do hieu gia virus dai. Doi tUgng va phtfang phap nghien ctfu: cdng hap dddc chi tao

theo phdang phap cua Ed HaHow, David Lane 1998, sd dung nen ti bio va cac virus sdi, quai bi, rubella,

virus dai de dinh gia chat Idang cua cdng hap. Phuang phip chuin do hidu gii virus dai tren chudt dddc sd

dung de dinh gia hieu qua cua cdng hap trong viec chuin do hieu gii virus dai tren ti bao thdng qua test -

T va Leven. Ket qua: cdng hap san xuit thd nghidm cd hi$u gii 1/80, on djnh d 4°C trong 30 thing, die

hieu vdi virus dai vi khdng cd FITC dd thda; hieu gii cua 116 loat virus dai chuin dd bang phdang phip

tren ti bao sd dung cdng hap san xuat thd nghiem cho kit qua tdang ddang vdi chuin do hidu gii tren

chudt vdi p > 0,05.

TO khoa: khang the, FITC, virus dai

I. DAT VAN DE

Phin flng mil n djeh huynh quang II ky thuat

vang trong giam sat phdng thf nghiem benh dai,

hdn nfla edn ed flng dung kh i rpng rai trpng

nghien cdu virus dai va kiem djnh d c cdng doan

trong qua trinh nghien cdu, san xua't vaccine dai.

Tren th i gidi da ed khing the da ddng, ddn ddng

khang virus dai gi n huynh quang (FITC) thUdng

mai hda cua hang Biorad. Sinh pham eua hang

Biorad ed gia thanh eao, hdn nfla sinh pham lai

khdng ludn sin ed trong nUdc va phu thupe rat

nhieu vao d c nha nhap khau. Chfnh vi vay viec

ehu dpng san xul't sinh pham trong nflde la dilu

can thilt, phuc vu cho nghien cflu, chan doln

benh dai cung nhU trong nghien eflu san xuat vac￾cine dai the he mdi. D l tai dUdc thflc hien vdi

d c muc tieu:

1. Nghien ctfu san xuat cong hgp khang the

khang dai gin huynh quang.

2. Lfng dung cong hgp san xuat thd nghiem

de chuan do hieu gia virus dai.

II. VAT LIEU VA PHUONG PHAP

NGHIEN CLfu

1. Vat lieu nghien cOu

IgG ehudt khing virus dai (lOmg/ml) da dUdc

tinh che do Vien Ve sinh Djch te Trung Udng

(VSDTTU) san xuat; Virus vaccine tam lien sdi,

quai bj, rubella (SMD); ehung virus CVS -

Chalenge Virus Strain; te bao BHK 21 (Phlp);

Khing the ddn ddng khing nucleoprotein eua vi￾rus dai gan huynh quang (Biorad); 116 loat virus

dai Vnukovo - 32 (VSDTTU); CIc dung cu, thie't bj

thflc hien d c ky thuat hoi sinh, mil n djch.

2. PhOdng phap nghien cOu

2.1. San xuat khang the khang virus dai gin

huynh quang -FITC

2.1.1. Quy trlnh san xuit

Quy trinh sin xuat khing th i khang virus dai

gan huynh quang FITC (Fluorescein Isothiocyanate)

dflde thflc hien theo phflpng phap eua Ed Harlow,

David Lane - 1988. Chuan bi tao cpng hpp: IgG

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