Quy trình phát hiện các chất sudan trong thực phẩm và mỹ phẩm bằng phương pháp HPLC

^ ^ighien CLFU - Ky thuat

r

Quy trinh phat hien cac chat sudan

trong thu'c pham va my pham

b3ng phu'ang phap HPLC

Dat van de

Sudan la mpt nhdm ehit mau bj elm su dung

trong thue phim va my phim do eo kha nang gay

ung thu. Tuy nhien, tren thj trudng ngudi ta da

phat hien mpt sd san phim ed ehua sudan vdi

mue dieh tao mau sle dep mdt, hip din ngudi su

dung'^' ^'. Mpt sd phdng thi nghiem eung dd vd

dang then khai nghien euu, phan tieh de phdt hien

sudan trong thue phim, my phim'^' ^' song khong

eong bd diy du quy trinh phdn tich va cac kit qua

thim djnh quy trinh. De phdt hien ede ehit mau

sudan blng quy trinh phu hgp vgi diiu kien phd

biin cua cae phdng kiem nghiem tai Viet Nam,

nhom nghien euu dd xay dung quy trinh phdt hien

eae chat sudan trong thue phim, my phim blng

sic ky Idng hieu nang eao (HPLC), detector UV￾VIS vdi ede hod ehit thdng dung, de kiim va re

nhu methanol, nude.

Thye nghiem

May moc, dung moi, hoa chit.

Nlay moc dung cu

- May sle ky Idng hieu ndng cao Shimadzu

vdi detector UV-VIS (phat hien ddng thdi tai 2

bude song 480nm vd 520 nm).

- Can phan tieh vdi dp ehinh xdc 0,1 mg.

Hoa chat, dung moi

- Methanol, aceton tinh khiit sdc ky.

- Cae chit chuan sudan: Cua cdng ty

Ehrenstori'er Quality (Cpng hod Lien bang Q(re).

Ddi tu'O'ng nghien cdeu

Bang 1: Ddi tugng nghien cuu

Loai mau Dang san

pham Loai san pham

Chi ke moi, ke mat

My pham

Dang ran

Dang sap

Dang long

Phin hong

Son moi

Son nuoc

San mong tay

TAP CHi DUQC HQC - 8/2010 (SO 412 NAM 50)

Nguyen Van Yen va CS

Sd Yte Ha Noi

Dang rin

Thuc phim

Bpt al, bpt gia vi

Bpt mau thuc pham

Tuong ot

Dang long Nuoc sot, goi lau

Trung

Phipcyng phap

Dieu kien sac ky

- Cpt: RP 18 (250 x 4,6 mm; 5 p.m).

- Pha dpng: methanol: nude (95:5).

- Tdc dp ddng: 1,5 ml/phOt

-Thetfeh tiem: 20^,1.

Detector: UV-VIS: X = -480 nm vdi sudan I vd

sudan 11. X = 520 nm vdi Sudan 111 vd sudan IV.

Chuan bi dung djch thw: Cdc miu thu dugc

xu ly theo quy trinh xir ly mau (da dugc ddng

trong Tap chi Dugc hpc thang 7/2010, tr. 18-23).

Chuan bi cac dung dich chuan

Bang 2: Cac dung djch chuin me

STT Sudan

1 Sudan 1

2 Sudan 11

3 Sudan III

4 Sudan IV

Khoi luang Dung moi

ean (g) hoa tan

0,0256

0,0261

0,0254

0,0255

Methanol

Methanol

Ethylacetat

Ethylacetat

Wong do

thu duac

(ppm)

230,40

234,90

243,84

234,60

De tien hanh lua chpn dieu kien sac ky, chiing

tdi tiin hdnh pha dung djch chuin tu dung djeh me

vdi dung mdi methanol : ethylacetat cd ndng dp

cac chit sudan (khoang 25 ppm): sudan I: 23,04

ppm; Sudan 11: 23,49 ppm; sudan 111: 24,384 ppm;

Sudan IV: 23,46 ppm.

Kit qua va bien luan

Danh gia tinh thich hap cua he thdng

Tinh thIch hgp cua he thdng sic ky dung de

xde djnh chdc chin ring dp phdn giai vd dd lap

lai ciia he thdng sle ky du d i tien hdnh qud

trinh phdn tieh.

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