Nhân giống in vitro hoa hồng môn (Anthurium andraeanum)

KHOA HOC C6NG NGHl

ixihA ixi g io ixig in V ITR O H O A HO IMG Mdixi

[Anthu rium and ra eanum )

Hoing Thi Nhu Phuong1, Trdn Tuin Dat1 Trin Via Minh2

t 6 m t At

Nh^n giong h6ng m6n in vitro qua nudi ciy tir miu la da dugc hoan thi£n d£ t?o ra m0t lugng ldn ciy con

ddng nhit trong mdt thdi gian ngin nhim dap ung nhu ciu v£ giong t^i dja phuong. Mdi trudng thich hgp

nhit cho qud trinh t$o md s^o tii md li la mdi trudng MS cd bd sung 1 mg/1 BA k£t hgp 0,5 mg/12,4-D cho

khi nang tao md s?o 83,33%. Khi nang tii sinh choi tir md s^o va nhan choi in vitro tot nhit tr£n mdi trudng

MS cd bo sung 3 mg/1 BA k£t hgp vdi 0,5 mg/1 NAA d^t kha nang tii sinh 25 chdi/miu vi nhan giong 3,9

chdi/miu. Mdi trudng MS cd bo sung 0,3 mg/1 IBA phit sinh t?o r£ li cao nhit 7,2 r£/ch6i. Gii tW phii

hgp nhit cho giai do?n vudn uom la triu, tro vd phan chudng (ty 1$ 4: 3: 3) d?t ty 1$ sdng sau dng nghidm li

100% sau 4 tuin.

Keywords: Anthurium andreanum, nudi ciy md, md s$o, c\tm chSi, g ii thS.

I.BATVANOt

Hong mdn (Anthurium andraeanum) li loai hoa

dugc nhi£u ngudi ira chupng vi chung khong nhung

li mpt loii hoa dpp, da dang ve hinh thii, miu sic,

mi c6n li loai hoa b<h vi tuoi liu. Hong mon li loii

Ion vi da dang hinh thii trong hp Aracaceae vi c6

1000 loii khic nhau. Hai loii kinh t£ dupe trong pho

btffn li Anthrunioum andraeanum vi A

scherzerianum vi cong nghidp hoa hong mon cit

cinh dupe thu hii tir cic ddng thuin hay dong lai [6].

Nhin giong truy&i thdng hong mon chu y£u dua

vio ky thuit tich choi, chia bui...tu ciy mp vi mit

nhi£u thdi gian d£ nhin gidng vdi sd lupng ldn cac

ddng d$c sic vi trdng tu hat thudng xuit hi£n nhung

thd h^ ciy lai d) hpp tir, hon nua, ty le nay mim thap

vi ty lg sdng thip [9]. Nhung phuong phip niy cdn

cd nhung han ch£ nhit dinh: sd lupng ciy nhin ra it,

khdng dam bao chit lupng cay con gidng vi thd

khdng du dip ung cho quy md san xuat ldn vi nhu

ciu cua thi trudng. Ky thuit vi nhin giong cho phep

nhin nhanh ci thd chpn lpc trong thdi gian ngin va

vin giGr dupe d$c didm di truy£n hon phuong phip

truydn thong [6]. Nhin gidng qua nudi ciy ddt thin

cd he sd nhin gidng thip [7], Ky thuit nudi ciy phat

sinh co quan da dugc ung dung thanh cong trong

nhin gidng hdng mdn [9,2,4,5,10,1,3].

O nude ta hien nay, cdng nghe nhin gidng ciy

hoa in vitro da dat dupe nhung thinh cdng nhit dinh

1 Dai hpc Da Lat

2 Dai hpc Quoc te, DHQG TP. HCM

tren mdt so ddi tupng nhu lily, cim chudng, cuc,...

Hdng mdn li mpt loii hoa mdi du nhip vio thj

trudng Viet Nam trong nhung nam gin diy nen cic

nghi£n cuu nhin gidng in vitro loii hoa niy nhim

chu dpng ve ngudn gidng cdn han che. Trong khi d6,

nhu ciu cua thi trudng ndi dia cua loai hoa niy ngiy

cing cao. Nhin nhanh in vitro ciy hdng mdn bing

ky thuit nudi ciy md thuc vit nhim muc tieu ning

cao nhanh he sd nhin gidng, giim chi phi sin xuit

vi chu ddng cung cip ngudn gidng cd chit lupng,

dip ung nhu ciu ngiy cing cao cua thi trudng.

2. VAT U|U VA PHUONG PHAP

2.i. vat lieu

Giong hoa hdng mdn (Anthurium andreanum)

cd miu dd mdi du nhip. Chpn nhung ciy sinh trudng

vi phit trien tdt, cho hoa dpp. Tidn hinh cit liy li d£

lam thi nghiem.

Mdi trudng dinh dudng co h a n : Murashige￾Skoog [8] cd bd sung cic chit dieu hoa s in h truong

thuc vat BA (6-benzylaminopurin), NAA (a￾naphthaleneaxetic axit), IBA (indol-3-butyric axit).

Cic thinh phin khic: dudng sucroza (30 g/T), aga (8

g/1). Ba loai gii the su dt^ng trong giai doan vudn

uom li triu: tro (6:4); triu: tro: xo dua (4:3:3); triu :

tro: phin chudng (4:3:3).

Mdi trudng dugc dieu chinh ve pH-5,8 truoc khi

hip khir trung trong autoclave d 1 atm, 121°C trong

20 phut

74 NdNG NGHlfP VA PHAT TRIEN NONG THON - KY2-THANG 4/2014