Nghiên cứu thành phần hóa học của cây Xáo leo (Paramignya Scandens)

TAP CHI HOA HOC

DOL 10.15625/0866-7144.2015-0093

T. 53(1)84-89 THANG 2 NAM 2015

NGHIEN CLPU THANH PHAN HOA HOC CUA CAY XAO LEO -

PARAMIGNYA SCANDENS

Nguyen Thi Dieu Thuan', Nguyin Hihi Toan Phan'*, Nong Van Duy', Ninh Thi Nggc\

Nguyin Van Thanh^ Nguyen Xuan Cudng^, Nguyen Hoai Nam\ Phan Van Kiem\ Chau Van Minh^

'Vi?n Nghiin cuu Khoa hgc Tdy Nguyen. Vi^n Hdn ldm Khoa hgc vd Cdng nghe Vi^t Nam,

^ Vi4n Hda sinh Bidn, Vien Hdn ldm Khoa hgc vd Cdng nghe Viet Nam

Din Tda soan 10-2014; Chip nhan dang 30-01-2015

Abstract

Phytochemical investigation of the methanol extract of Paramignya scandens led to isolation of seven compounds

including: a limonoid methyl isolimonate (1); a flavonone diglycoside atripliside B (2); a phenyl derivative trans-ti-p￾coumaroyl tyramine (3); two megastigmane glycosides gusanlungionoside C (4) and (6/i,95)-roseoside (5); a lignan

glycoside syringaresinol di-0-/3-D-glucopyranoside (6); and a nucleoside adenosine (7). Their structures were elucidated

by ID and 2D NMR experiments. This is the fust report of these compounds from P. scandens.

Keywords. Paramignya scandens, Rutaceae, limonoid, flavonone diglycoside, megastigmane, lignin.

1. MO DAU

Xao leo - Paramignya scandens (Griff.) Craib, la

cay day leo nhd hoac trudn thugc chi Paramignya

Wight, hg Rutaceae. O nude ta, loai nay thudng thiy

d Nghe An, Quang Tri, Lam Ddng va D6ng Nai [I,

2]. Den nay, chua cd nhilu cdng trinh nghien cuu vl

thanh phan hda hgc va boat tinh sinh hpc ciia cac

loai thugc chi Paramignya dugc cdng bd. Mdt vai

cdng trinh da cdng bd cho thiy cac loai Paramignya

cd chiia cac hgp chit tritecpen dang khung tirucallan

[3-5], courmarin [6, 7] va flavonoit [4, 5]. Trong

cdng trinh nay, chiing tdi c6ng bo qua trinh phan lap

va xac dinh cau true ciia bay hpp chat phan lap duoc

tiir eay Xao leo - P. scandens la: methyl isolimonate

(1), atripliside B (2), /rflni-N-/7-coumaroyl tyramine

(3), gusanlungionoside C (4), (6fi,95)-roseoside (5),

syringaresinol di-O-^D-gluco-pyranoside (6), va

adenosine (7). Day la lin dau tien cac hgp chit nay

dugc phat hien tir cay xao leo.

2. THUC NGHIEM

2.1. Phuong phap va thiet hi

Ph6 'H-NM R (500 MHz) va '^C-NMR (125

MHz) dugc do tren may Bruker AM500 FT-NMR,

TMS dugc sir dung lam chit chuan ngi. Phd khli

lugng (ESI va APCI-MS) dugc do tren may Agilent

1260 series single quadrupole LC/MS. Sic ky cgt

(CC) dugc tien hanh vdi chit hip phu la silica gel

(Kieselgel 60, 70-230 mesh and 230-^00 mesh,

Merck) va YMC RP-18 (30-50 ^m, Fuji Silysia

Chemical Ltd.). Sic ky ldp mdng (TLC) sir dyng

ban mdng trang sin silica gel 60 F254 (1.05554.0001,

Merck) va RP-18 F2S4S , plates (LI5685.000I,

Merck). Vet chit dugc bien thj bing each phun

thude thu axit H2SO4 10% va ho ndng tir tir den khi

hien mau.

2.2. Mau thuc v^t

Mau cay Xao leo - Paramignya scandens

(Griff.) Craib, thupc hg Cam quyt - Rutaceae, dugc

thu thap t^i Da Lat, Lam Ddng trong thang 5 nam

2013. Ten khoa hgc dugc TS. Ndng Van Duy giam

dinh. Miu tieu ban (sd TN3/055) dugc luu tai phdng

miu Clia Vien Nghien cuu Khoa hpc Tay Nguyen.

2.3. Phan lap cac chat

Canh va la khd cay xao leo - P. scandens (1,5

kg) dugc nghiin nhd va chilt 3 lin vdi MeOH

(3x5L) d nhi?t do tir 30-45 °C sii dyng thiit bj sieu

am. Dich chiet thu dugc dugc loai bd dung mdi dudi

ap suat giam thu dugc 85 g can chilt. C?n nay dugc

hda vao nude cit va chilt phan bd lin lugt vdi cac

dung mdi n-hexan va CH2CI2 thu dugc cac can chilt

tuang ung: «-hexan (PSh, 15,2 g), CH2CI2 (PSc,

37,3 g) va ldp nude (PSw).

Can CH2CI2 (PSc, 37,3 g) dugc phan tach thanh

08 phan doan (FSc-I din FSc-8) bing sic ky cot