Nghiên cứu thành phần chất màu anthocyanin chiết từ quả dâu tằm Quảng Nam

Tap chi Hoa hgc, T. 48 (4), Tr. 491 - 496, 2010

NGHIEN cufu THANH PHAN CHAT MAU ANTHOCYANIN

CHIET TLf QUA DAU TAM QUANG NAM

Den Tda sogn 17-9-2009

D A O HUN G CUdNG, HUYNH THI KIM CUC

Khoa Hoa hgc, Trudng Dgi hgc Suphgm - Dgi hgc Dd Ndng

ABSTRACT

The method to identify the composition of anthocyanin coloured compound extracted from

mulberry fruits (morus alba) in Quang Nam province in mixed solvent ethanollwater (III, 1%

HCl) by using liquid chromatography - mass spectrometry LCQ-Finigan was introduced. The

mobile phase consists of water, methanol and acetonitril 1% acetic acid with the proportion

30:60:10, with aftow rate at 0.7 mllmin; mass range: 10 - 1000; scan wavelength 400 - 600 nm;

combining with the analysis of segment changes based on chromatography. The obtained results

shown that three peaks of three molecules ions, with the saving moments at 2.37; 3.02 and 3.10

min, identified with heavy weight at mIz = 306.8; 595.1 and 449.0 are chloride of pelagonidin,

cyanidin-3-O-glucoside and cyanidin-3-0-p-coumarylglucoside, respectively.

1-DATVANDE

Anthocyanin la nhiing glucozit do gdc

dudng glucose, galactose... kit hgp vdi gdc

aglucon cd mau (anthocyanidin). Aglucon cua

chiing cd ciu true ca ban dugc md ta trong hinh

1. CIc gdc dudng thudng dugc gin vao vi trf

cacbon 3 va 5, trong mdt sd tradng hgp d

cacbon 7. Phan tit anthocyanin gin dudng vao

vi trf 3 ggi II monoglycozit, d vi trf 3 vl 5 ggi II

diglycozit. Cac aglucon cita anthocyanin khic

nhau chfnh la do su cd mat cita cac nhdm Rj va

R2 khac nhau, cle nhdm nay thuang la H, OH

hoac OCH3 [5].

Anthocyanin chiit tich tit cac loai thuc vat

khac nhau thi cd nhiing dac tfnh khac nhau, dac

biet II dd bin mau dudi tic dung cita cle diiu

kien cdng nghe. Su khic biet ve mau sic dd

chfnh la do thanh phin khdng gidng nhau trong

td hgp mau cita anthocyanin gay nen trong dd

cd anthocyanin trong qua dau (Mulberry) ndi

chung va qua dau tim cita Quang Nam ndi

rieng. Chfnh vi vay chiing tdi tiin hanh nghien

ciiu dinh danh thInh phin cita anthocyanin

trong chat mau chiit tit qua diu tam Quang

Nam di llm ca sd cho viec ting diang loai phim

mau nay trong chi biin thuc pham cd gia tri va

hieu qua tdt hon.

Hinh 1: Cau tnic ca ban cita aglucon cita

anthocyanin

11 - DOI TUONG VA PHUONG PHA P

NGHIEN CUU

1. Ddi tirgng nghien ciru

491