Kết quả ban đầu về thành phần hóa học cao chiết diclometan lá cúc quỳ (Tithonia diversifolia (hemsl.) A.gray)

TAP CHI HOA HOC 1.51(6)770-773 IHAMLi IZ INAJVl ZUIJ

K^T QUA BAN DAU VE THANH PHAN HOA HOC CAO CHIET

DICLOMETAN LA CUC QUY

(TITHONIA DIVERSIFOLIA (HEMSL.) A. GRAY)

Tran Thi Thanh Thuy'', Tran Van Hilu', Vii Thi Thu Ha', Trinh Thi Thiiy^ Tr^n Van Sung^

'Phdng Thi nghiem trgng diem Cdng nghe loc, hda ddu. Viin Hda hoc Cdng nghiep Viet Nam

' Vien Hda hoc, Vien Hdn ldm Khoa hoc vd Cdng nghi Viet Nam

Din Tda soan 15-10-2013

Abstract

Four compounds: mynstyl myristate (1), hispidulin (2), eupafolm (3) and tagitimn A (4) were isolated from the

dichlomethane leaf exUact of Tithonia diversifolia (Hemsl.) A, Gray, Their structures were elucidated by analysis of IR,

MS, and NMR spectra and companson with the published data. Compounds 1 and 3 were isolated for the time from

this plant,

Key>vords: Tithonia diversifolia, Asteraceae, hispidulin, eupafolm, tagitinin A.

1. DAT VAN DE

Ciic quy hay cdn ggi da quy, son quy bien diep,

quy dai, hudng duong dai, hudng duong Mexico...

{Tithonia diversifolia) la mdt loai thuc vat trong hp

Cue (Asteraceae) cd ngudn gdc tir Mexico, hien nay

dugc phan bd rdng khap d cac khu vuc can nhiet ddi

va nhiet ddi [1], Ciic quy thupc dang cay bui, cao 1-

2 m, than cd long, phan thanh nhieu canh, la thudn,

phien CO thiiy, bia cd rang. Hoa d dau nggn, tren

cudng dai. Hoa d bia hinh mdi lep, vang tuoi; hoa

giira hinh ong; giira hoa cd vay. Cay ra hoa tir thang

12 den thang 2. 0 Viet Nam, cay ciic quy hien nay

da duoc tu nhien hda, mgc phd bien d rat nhieu noi,

dac biet la d viing Tay Nguyen [2].

Trong y hoc cd truyen Chau My, nudc ep tu re va

la cay dugc su dung dieu tn ap-xe [3]; d Mexico loai

nay dugc uong de chua sdt ret va dien hinh la chua

cac khoi tu mau va riit co, bpt tir la sao khd tri vet

tham, vet thuang va viing da nhiem triing,,.. Ci Dai

Loan, dich chiet nudc ciia la cay dupc sii dung de trj

dai thao dudng (chimg dai nhieu va chimg khat

nhieu) [4] va viec ung dung ciia T diversifolia trong

dieu Iri dai thao dudng cung da duoc dang ki bao

hd[5],

Tren the gidi, cay cue quy dugc quan tam nghien

ciru ve thanh phan hda hgc va hoat tinh nhilu nhat

hong cac loai thuc vat thudc chi Tithonia [10]. Cac

hgp chat sesquiterpen lacton va ditecpen la thanh

phan hda hgc chinh va cd nhieu hoat tinh thii vi ciia

loai nay [10]. Tuy nhien, cho den nay, chua cd cong

trinh nao ve thanh phan hda hgc ciia cay ciic quy thu

hai tai Viet Nam dugc cdng bd. Bai bao nay cdng bo

ket qua budc dau ve viec khao sat thanh phan hoa

hpc ciia cao chiet diclometan tir la cay ciic quy thu

hai tai Tay Nguyen.

2. THUC NGHIEM

2.1. Phuo'ng phap nghien cuu

Sac ky ban mdng dugc tien hanh hren ban silica

gel Merck 6OF354. Sac ky cdt sii dung silica gel cd

hat 0,04-0,063 mm.

Phd cgng hudng tir hat nhan NMR dugc ghi tren

may Bruker Avance 500 MHz, phd khdi ESI-MS

dugc do tren may Water LC/MS/MS-Xevo TQ. Phi

IR dugc ghi tren may Nicolet 6700 FT-IR.

2.2. Mau thuc vat

Mau thuc vat dugc thu hai tai tinh Gia Lai vao

thang 4 nam 2012. Tieu ban dugc luu giir tai Phong

Thi nghiem trgng diem Cdng nghe lpc hda diu. Mau

la dugc rira sach, phai, say khd.

2.3. Thuc nghiem

La ciic quy (400 g) da say khd, xay nho dugc

chiet nhanh (khoang 3 phut) bang diclometan (x

3Ian) d nhiet do phdng theo qui trinh trgng tai li?u

[13]. Lgc dich chiet, loai dung mdi dudi ap suat

giam. Can cdn lai dugc hda tan trong MeOH-H20

(7'3, v:v) va chilt phan ldp lan lugt vdi «-hexan va