HPLC A Praactical User''''S Guide Part 8 ppt

Commercial mobile phase recyclers use peak detection from either a com￾puter or an integrator to signal baseline acquisition. A waste/recycle valve is

triggered by this signal to discard peak containing mobile phase and recycle

the baseline. There is little solvent recovery for chromatograms containing

many closely spaced peaks, but for normal chromatography or in cases where

the HPLC is always left running this might represent 40–50% solvent recov￾ery. Estimates have appeared in the literature that claim that for a quality

control instrument run on a single shift this would mean a savings of about

60 L per year at a savings of about $3,000/yr in solvent, man power, and envi￾ronmental impact. Obviously, the savings would be higher in stat instruments

running in clinical laboratories and in quality control laboratories that operate

on a three-shift schedule.

134 TROUBLESHOOTING AND OPTIMIZATION

III

HPLC UTILIZATION