Giá trị của SCC - AG/huyết thanh của các bệnh nhân ung thư cổ tử cung trước, trong và sau xạ trị

TCNCYH 69 (4) - 2010

9. Sanjoy K.B, Shyam Ramchandani, Nadia 11. Winston S.N. Shim, Shujia ), Philip W,

Cervoni, Moshe Szyf. (1999). "A mammalian Jack T, Yeow L.C (2004)."Ex vivo diffentiation of

protein with specific demethylase activity for human adult bone marrow stem cells into

mCpG DNA". Nature 397, 579 - 583. cardiomyocyte - like cells".

10. Tomita S, Li R.K, Weisel R.D, Mickle D.A, 12. Xu W, Zang X, Qian H, Zhu W, Sun X, Hu

Kim E.J, Sakai T, Jia Z.Q. (1999). "Autologous J et al. (2004), "Mesenchymal Stem Cells from

transplantation bone marrow cells improves Adult Human Bone Marrow Differentiate into a

damaged heart function". Circulation 100: Cardiomyocyte Phenotype In Vitro". Biol Med

247 - 256. ..... , 229: 623 -631 .

, . J Summary

USIN G 5 - AZACYTIDIN E INDUCE D MESENCHYMA L STE M CELLS FROM HUMA N

BON E MARRO W TO DIFFERENTIATE TORV^ARD CARDIOMYOCYTE S IN VITRO

Heart is high differential tissue so that the regenaration df adult cardiumydcytes is limited, specially

affter myocardial infarction. Cell tharepy includes mesenchymal stem cells with their potency has become

an increasing field of interest. Objective: (l)Appling a protocdl use 5 - azacytidine induced mesenchymal

stem cells from human bone marrow to differentiate torward into cardiomyocytes in vitro. (2)lnvestigating

a successful level of this protocol that differentes MSCs toward cardiomyocyte - like cells. Method: In vitro

model. Mesenchymal stem cells from human bone marrow at passeges 2 were induced by 5 - azacytidine

lOpM in 24 hours, were colected to exam affter that about 3 - 4 weeks. Using RT - PCR,

immunohistochemistry (IHC) and transmission electren microscopy (TEM) to appreciate the results of the

differentiation process. Concludes:(1) 5 - azacytidin induced MSCs towards a cardiomyogenic lineage in

vitro. (2) Results of defferentiation process are observated in three myo - cardiomyocyte - specific levels.

The induced cells increated gens expression (MEF2A, 2C, 2D va GATA4); enhanced skeleton cytoplasm

protein expression - Desmin, appeared intermediate filamens in cytoplasm, but not sarcomeres.

Keywords: 5 - azacytidine, mesenchymal stem cells

GIA TR! CUA sec - AG/HUYET THANH CUA CAC BENH NHAN

UNG THU CO TLf CUNG TRU6C , TRONG VA SAU XA TRI

Bui Dieu

Benh vien K

Muc tieu: danh gia nong do SCO - Ag tren nhOng benh nhan ung thu cd td cung trudc, trong va sau dieu

tri va tren ca sd dd cd the theo ddi tai phat va di can ciia ung thu cd td cung sau diiu tri. f>oi taang vk

phaang phap nghien ciiu: nghien cdu dugc thuc hien tren 70 benh nhan ung thu cd td cung tai benh vien K

trong 2 nam tU 2008 - 2009, nhOng benh nhan nay dugc theo ddi dgc cac tridu chUng lam sang va xet

nghiem nong do SCC - Ag/ huyet thanh. Ket qua: nong do SCC - Ag huyet thanh giam nhanh trong va sau

diiu tri, dong hanh vdi ket qua dieu tri cua benh nhan. Ty Id benh nhan cd ndng do SCC - Ag huyet thanh

tU 2 ng/ml trd xudng (gia tri binh thudng) tang theo thdi gian, cao nhat d 3 thang sau diiu tri (88,8%o) va van

duy tri d mUc cao 6 thang sau diiu tri (88,3%o). Ket luan: nong do SCC - Ag huyet thanh la rat cd gia tri trong

viee theo ddi ket qua dieu tri ung thu cd td eung.

Jd khoa: SCC - Ag huyet thanh, ung thU cd tu' cung

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