Cảm biến sinh học sử dụng hạt nano từ

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CAM BIEN SINH HOC SVDUNG HAT NANO TV

A B106ENS0D

EMPIOYING MAGNETIC

NANOPACTICLEA

Summary J Using magnetic nanoparticles

as labeling agents for detecting

and quanUlying the level of

biological molecules are of

interest in many biomedical

applications recently. Owning

to the high biocompatibility of

their surfaces, the magnetic

nanoparticles have the ability

to conjugate specifically to

the biological molecules

of interest. Because of

such specific binding, the

concentration of the biological

agent is determined by number

of magnetic nanoparticles. A

sensitive magneto-biosensor

using giant magneto￾resistance sensing element

together with a new sensing

technique can determine

accurately the number of

the magnetic nanoparticles

via a measurement of their

induced magnetic field. It

was demonstrated that the

technique can help to detect

and measure low biological

concentrations in a timely

fashion without the need of

a speciat magnetic-shielding

medium.

TS CAO XUA N HO U

Tnfcmg Ba\ hoc S>ich khoa, Pai hoc Bh Hinq

i.Md'dau

Trong y sinh hpc ndi chung, viec xac djnh ham lupng cac thgc

the sinh hpc cd sai hong, bj nhidm benh hay ed nguy cd phd hQy

eao trong mpt dung djch la het sQe can thidt. Tuy nhidn, cho tdi nay

cdc phUdng phap truyen thdng phue vg do ham lupng cdc hogt chdt

ndy thudng bj gidi han bfli rat nhidu yd'u td nhu dp nhgy, thdi gian

do, kha nang phdn tich da chdt, hay sU phdn Qng khd khd'ng ehd vdi

thdnh phan phQc tgp cua mdu sinh hpc. Cdc phUdng phap ndi ehung

ddu sQ dgng mdt bd chuyen doi tin hieu de thu nhan sg cd mat cua

nhQng lien ke't giQa phan tQ sinh hpc (PTSH) va phan tQ nhdn bidt

dde hidu. 6 eac phUPng phap nhu ELISA [1], thu nhdn lupng tQ, hay

sQ dung mdng kich thude micromet [2, 3], trj sd do fl ldi ra cija bO

chuyen ddi dupe xdc djnh dga tren tin hieu do mdu sdc hoac tin hidu

phat quang cua mdu. Vl the, mQc dp hap thg quang hpc hoae khd

nang tg phat quang ndi tgi cua nhieu mau sinh hpc se gay nham ldn

kd't qud do va tao ra gidi hgn eho eae phep do nay. TUdng tg nhg

vay, cac cam bidn sinh hpe sQ dung phan tQ nhgy la ddy nano [4],

dng eacbon [5], can nang micro [6], hay cdm bie'n didn hda [7] Igi

dga vao cac tQdng tac dien tQ giQa protein hoac ADN vdi phan tQ

cdm bidn. Didu nay lam gidm mQe dp tin eay eiia eae phep do khi

tien hanh vdi eae dung djch cd dp pH hodc ndng dO ion bidn ddi.

Trong khoang mOt thap nien trfl lai day, phQdng phap do hdm

IQpng hogt chat sinh hpc sQ dung cac tdc nhdn tQ tinh dQpe quan

tdm rdng rai do tinh Qng dgng thue tien ldn. Gan ddy, edc hgt nano

tQ tinh ed kieh thude 5-150 nm da dupe thQ nghiem va cho tha'y khd

nang Qng dung to Idn cua ehung trong cac Qng dgng nhU ddnh ddu

sinh hpe, ddn truyen thudc, ddt nhiet tQ hay lam tac nhan tang dp

tQdng phan cho anh cdng hQflng tQ [8], Didu nay cd dupe Id nhd cae

lien kdt hda sinh dac hieu giQa eae PTSH (d thd tu nhien hoae nhan

tgo) vdi eae hgt nano tQ [9]. Qua khao sdt eho thay, cdc tin hieu tQ

tinh neu ed eua eae phan tQ nhQ protein hoac ADN khong bj cdm

Qng trong tQ trUdng ngoai, hay ndi each khac, khdng giao thoa vdi

ed che chuyen ddi tQ tinh cQa bp earn bidn tQ. Do dd, phUdng phdp

thu nhan va do dac sQ dgng tQ trudng rat phii hdp vdi viec do dgc

ede ham lUdng hogt chat nay trong eac mdu y sinh [10]. Ngoai dde

tinh phi tQ hoac khdng giao thoa tQ neu tren, sg hap ddn cQa phQdng

phdp con the hien fl kha ndng khdng phd hQy trgng thai sinh hpc

eiia nhidu he ly sinh khac nhau [11] so vdi cdc phUdng phap truydn

thdng khac. Hdn nOa, theo tinh todn, khi eac hat nano hodc micro tQ

50 TapchiKHOAHQC ^.^ ^

CONGNCHtVIETNiftf SSen.m2013