Xác định 3 phân Typ P-ALA, P-THR và V-VAL của EBNA-1 ở virus Epstein - Barr phân lập tại Việt Nam

TCNCYH 70 (5) - 2010

Summary

AUTOLOGOUS PERIPHERAL BLOOD STEM CELL CARRIED - OUT AT THE CENTRAL

MILITARY HOSPITAL 108 FOR THE PATIENTS WITH NON - HODGKIN LYMPHOMA

AND MULTIPLE MYELOMA

High - dose chemotherapy with autologous peripheral blood stem eell(PBSC) transplantation is the front

- line treatment for certain patients with non - Hodgkin's lymphoma (NHL) and multiple iTiyeloma(MM).

Objectives: applying the autologous PBSC transplantation in treatment of the patient with NHL and MM,

evaluating its safety and effectiveness. Method: PBSCs of the NHL and M M patients being in remission

were mobilized by the combination of Cyelophosphai-nide(2 - 4g/m') and G - CSF (5-1 0 mg/kg/day);

PBSCs were collected and eryopreserved in - 196°C; BEAM and high - dose Melphalan were used as the

pre - transplant conditioning regimens for NHL and M M patients respectively; autologous PBSC transplan￾tations were performed and followed - up. Results: the mean time of PBSC mobilization was 10.25 ± 1.7

days; the mean CD34-I- cell count collected by 3 - 4 consecutive apheresises was 6.95 ± 2.60 x lOVkg;

the cell viable rate of the PBSC eryopreserved in - 196''C was approximately 90%; PBSC transplantation

was carried - out safely with the rapid engraftment and hematopoietic recovery (the mean times of

granulocyte and platelet recovery were 11.0 ± 0.9 days and 11.8 ± 1.6 days respectively); the event - free

survival of the NHL patients after transplantation was longer in comparison with that one of M M patients.

Conclusion: autologous PBSC transplantation was performed safely and effectively.

Keywords: peripheral bood stem cell (PBSC), autologous PBSC transplantation, non - Hodgkln's lym￾phoma (NHL), multiple myeloma (MM)

XAC DjNH 3 PHAN TYP P - ALA, P - THR VA V - VAL CUA EBNA - 1

6 VIRUS EPSTEIN - BARR PHAN LAP TAI VIET NAM

Le Thanh Hoa', Vu Thi Tie'ng Hoang Thj Mlnh Chau', Le Thanh Ha^ Nguyin Dinh Phuc^

'Vien Cdng nghe sinh hoc; 'Hoc vien Quan y; ^Trddng Dai hoc Y Ha Noi

EBNA - 1 lien quan mat thie't de'n ung thd vdm mui hong (NPC) va tang nhiim virus. Da hinh tai vi tri

acid amin 487 cho phep xic dinh 5 phin typ: P - ala, P - thr, V - vai, V - leu, V - pro (P: prototype; V:

Variant): Can lim sing td trong miu benh pham NPC cua benh nhin Viet Nam cd bao nhieu phin typ, thudc

loai nao. Muc tieu: phit hien va xac djnh phin typ EBV bang phdang phip phan tich dac diem phin td

EBNA - 1. Doi tugng va phuang phap nghien ciiu: tach DNA, thdc hien PCR, thu nhin doan gen EBNA - 1

(0,76 kb); giii trlnh td, so sanh; phin tich acid amin EBNA - /, nhan dinh da hinh tai vi tri 487 de xac djnh

phan typ tren 22 miu bdnh phim sinh thiet td benh nhan mic NPC nhip vien 4 vung, gom Hi Noi (6 miu),

Hai Phdng (6 miu), Di Ning (3 miu) vi TP Ho Chi Minh (7 miu). Ket qua: trong sd 22 miu NPC cua Viet

Nam phit hien 3 phin typ: P - ala, P - thr vi V - vai, chda phit hien V - leu va V - pro. Ha Ndi cd 4 miu V -

vai; 2 mau P - thr; Hai Phdng cd 4 V - vai, IP- ala, 1 P - thr; Da Ning cd 2 V - vai, IP- thr; TP hid Chi Minh cd

4 V - vai, 2 P - ala, ? P - thr. Ket luan: ba phin typ P - ala, P - thr vi V- vai Ion tai trong sd 22 miu NPC, ngoai

trd P - ala chda phit hien d Hi Ndi, 3 vung cdn lai deu chifa ca 3 phin typ. Phin typ V - vai gap d tat ca benh

pham, ddac xic nhin cd the cd vai trd chinh trong tien tnen ung thd (tumorigenesis) cua NPC. Sing loc phan td

xic djnh phin typ EBNA - 1 li can thiet vl cd the giup cho djnh hddng chan doin sdm va phdng chdng NPC .

TO khoa: EBV, NPC, EBNA - 1, PCR, P - ala, P - thr, V - vai, V - leu, V - pro