Thích nghi chủng sản xuất Vaccine dại vnukovo - 32 trên tế bào vero

TCNCYH 65 (6) - 2009

THICH NGHI CHUNG SAN XUAT

VACCINE DAI VNUKOVO - 32 TREN TE BAO VERO

Nguygn Thj Kieu Anh, Ngo Chau Giang,

Nguygn VTnh Dong, Nguyen Thj Hong Hanh

Vien ve sinh Dich td Trung Uang

De tien tdi sin xua't vaccine dai te bio trong nUdc thi van de mau chdt phai cd chimg sin xuat cd chat

luang cao. Muc tieu: thich nghi chiing sin xuat vaccine dai Vnukovo - 32 tren te bio Vero. Bdi tugng va

pbuang pbap ngbien cdu: thich nghi chimg virus bing phuang phap cay truyen lien tiep. Ket qua, ban luan:

Chiing Vnukovo - V06 di hoin toin thich nghi tren te bio Vero d ddi ca'y truyen thif 6, cd bleu gii 10"

''LDsc/nil, dat cic tieu chuan chimg san xua't vaccine dai ciia TCYTTG ve tinh dn dinh hieu gia, vd khuan,

khdng nhiim mycoplasma, khdng nhidm virus ngoai lal, giim dgc lUc, cd dinh vi khi nang sinh miin dich li

0,97IU/ml. Ket luan: chiing Vnukovo - V06 li chiing Vnukovo - 32 di thich nghi tren td bao Vero va dat tieu

chuan chung sin xuat vaccine dai tren te bao Vero ciia TCYYTG.

Tff khoa: Chung Vnukovo - 32, te' bao Vero, thich nghi

LDATVAND E

Benh dai la benh viem nao tuy do virus dai gay

nen, Xy le tfl vong gin nhfl 1 00%, tuy vay benh cd

the phdng va diiu trj dfl phdng blng vaccine hoac

phd'i hpp vaccine vdi huyet thanh khlng dai.

Vaccine dai t l bao da dfldc chflng minh cd dp an

toan va kha nang bio ve cao dflpc TCYTTG khuyen

dung. D l tie'n tdi san xuit vaccine dai te bao trong

nfldc, chung tdi tie'n hanh nghien cflu vdi muc tieu:

Thich nghi cbung san xuat vaccine dai

Vnukovo - 32 tren td bao Vero nham tao chiing

gde (MSV) va chiing san xuat ('VVSVj; kiem tra

chat luang chiing gidng.

II. VAT LIEU vA PHUONG PHAP

NGHIEN CL/U

1. Vat lieu

- Chung san xuat vaccine dai Vnukovo - 32 (Vien

Bai liet va Viem nao Mosco); t l bao Vero (TCYTTG -

ECACC); t l bio CER, BHK, Na. (Nhat Ban); MEM

(Gibco); DMEM (Gibco); Trypsine (Gibco), mdi

trfldng cit gifl t l bao (Gibco); khlng the ddn ddng

(mab) khang N virus dai gan FITC (Mab - FITC)

(Bio - rad), PCR mycoplasma kit (Bioscience); huyet

thanh khlng dai (Pasteur Merieux).

* - Ddng vat thf nghiem: Chudt nhat, chudt d,

chudt lang (Cimade, vien VSDTTUl.

2. Phffdng phap nghien cffu

2.1. Thfch nghi cbung Vnukovo - 32 bang

phtfang phip cay truyen lien tiep:

c i y chung Vnukovo - 32 tren te bi o Vero d

diiu kien tdi flU vdi ndng dp nudi cay (MOI) la

0,3; pH 7,4; nhiet dp 37°C. Thu hoach virus d pha

phat triin ham sd mu [2], tii p tuc cay truyin de'n

khi hieu gi l dat lO^LDso/ml (ddi n) va dn djnh

hieu gia d hai ddi ci y truyin tiep theo. Sfl dung

ddi n lam chung gid'ng gd'c (MSV), ddi n-i-1 lam

chung gid'ng sin xuit (WSV), ddi n-f2 la virus sin

xuit vaccine.

2.2. Kiem tra cha't Idang chimg thfch nghi

theo htfdng din sin xuat vaccine vi cac che

pham sinh hgc cua Hoa Ky va TCYTTG:

- Kiem tra hieu gii virus: sfl dung hai phfldng

phap tren te bad va tren chudt

- Kiem tra nhan dang virus: phfldng phap hien