The analysis of triclosan in toothpaste

The Analysis of Triclosan in Toothpaste

via LC/FT-IR Mobile Phase Elimination AN-20

Introduction:

Triclosan (Figure 1), an anti-bacterial agent used to prevent gum disease, was qualitatively analyzed in toothpaste using

liquid chromatography for separation and FT-IR for identification. An LC-Transform interface was used to remove the

HPLC mobile phase and collect the sample for FT-IR analysis. The determination of components in dental and other

cosmetic products is useful and often mandated by the manufacturer and by regulatory agencies. In this case the positive

identification of triclosan in every batch of toothpaste is necessary as governed by good manufacturing practices (GMP).

HPLC-FTIR can replace wet chemistry methods as a faster, simpler means of identification.

Figure 1.

Experimental Conditions:

Chromatography (Waters 600-MS)

Column: Nova-Pak® C18 3.9 x 150 mm (Waters)

Mobile Phase: 60/40/0.5 acetonitrile/water/acetic acid

Column Flow: 1 mL/min

Injection Volume: Varied

Concentration: 83.4 mg/mL total toothpaste

UV detection (Kratos Spectroflow 757)

280 nm

LC-Transform Model 102 (Lab Connections)

Sheath Temperature: 100 °C

Sheath Gas Flow: 25 (units)

Nebulizer Flow: 32 (units)

The Analysis of Triclosan in Toothpaste

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