Taxonomy And Biology Of Calonectria In Forest Soil Of Vietnam

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ACKNOWLEDGEMENTS

In order to complete this study course, with the consent of the Department of Forest

Resources and Environment Management (FREM), I conduct to do a research: “Taxonomy

and biology of Calonectria in forest soil of Vietnam”.

In the implementing research process, I have received the helps of Forest Protection

Research Centre (FPRC) - Vietnamese Academy of Forest Sciences (VAFS), everyone who

work in this centre, and especially the enthusiastic guidance of Associated Professor Ph.D

Phung Van Khoa - Vietnam National University of Forestry and Associate Professor Ph.D

Pham Quang Thu - Forest Protection Research Centre.

Therefore, I would like to express deep gratitude to Associated Professor Ph.D Phung

Van Khoa, Associated Professor Ph.D Pham Quang Thu who guide for me. Thanks to Forest

Protection Research Centre, and all members in here who have created favorable conditions for

me to complete this study. I also have a good eye for helping of my family, and my friends.

In the process of learning and doing research, I tried my best but time, knowledge, and

budget are limited. Therefore, this study could not avoid some faults. I am looking forward to

hearing comments from you to more complete the study.

Sincerely!

Hanoi, September- 2016

Nguyen Thi Loan

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ABSTRACT

The research presents taxonomy and biology of Calonectria in forest soil of Vietnam.

Basing on data of 72 soil samples which were collected from some forests of Vietnam such as

Hoang Lien National Park, Tam Dao National Park, and Binh Phuoc province, the study

clearly described biology characteristics and pathogenicity of Calonectria. Furthermore,

taxonomy also is an important part of this research. Using method of sample collection in

collecting soil samples; method of isolation, culturing the fungus in finding fungi phylogeny;

method of pathogenicity in defining pathogenic ability; method of DNA technique in fungi

identification; method of using microscope in describing morphological characteristics of

fungi; and methods of culturing the fungus in different environment, temperature, moisture to

determine the biological characteristics of fungi. Data was collected in 2016 from 72 soil

samples in Hoang Lien National Park, Tam Dao National Park, and Binh Phuoc province.

The results of the study showed morphological characteristics and biological characteristics

of 41 isolates- not only this but also they were classified. Generally, this pathogenic fungi

need to be paid more attention in order to protect forest trees.

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TABLE OF CONTENTS

ACKNOWLEDGEMENTS

ABSTRACT

TABLE OF CONTENTS

LIST OF FIGURES

LIST OF TABLES

1. INTRODUCTION ............................................................................................................ 1

1.1. Imperativeness ............................................................................................................... 1

1.2. Study site ....................................................................................................................... 1

1.3. Object of research .......................................................................................................... 5

1.4. Literature reviews .......................................................................................................... 5

2. GOALS AND (SPECIFIC) OBJECTIVES ........................................................................ 8

2.1. Goals .............................................................................................................................. 8

2.2. Specific objectives .......................................................................................................... 8

3. METHODS ....................................................................................................................... 9

3.1. Sampling methods: ......................................................................................................... 9

3.2. Isolation methods: ........................................................................................................ 10

3.3. Methods of pathogenicity testing: ................................................................................. 11

3.4. DNA technique: PCR amplification and sequencing: .................................................... 13

3.5. Method of describing the morphological characteristics of fungi: ................................. 14

3.6. Method of describing the biological characteristics of fungi: ........................................ 15

3.6.1. Growth of the mycelium on different nutrient media: ................................................. 15

3.6.2. Growth of the mycelium on PDA nutrient medium at different temperature scales:.... 16

3.6.3. Growth of the mycelium on PDA nutrient medium at different moisture scales: ........ 17

4. RESULTS ....................................................................................................................... 18

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4.1. Result of sample collection. .......................................................................................... 18

4.2. Result of isolation, and culturing the fungus. ................................................................ 19

4.3. Pathogenicity testing. ................................................................................................... 22

4.4. Taxonomy by DNA technique: PCR amplification and sequencing. .............................. 24

4.5. Morphological characteristics of fungi. ......................................................................... 28

4.5.1. Morphological characteristics of Ca. follicola (DMC TN2.1) ..................................... 28

4.5.2. Morphological characteristics of Ca. pentaseptata (RTN BPP3.3) .............................. 28

4.5.3. Morphological characteristics of Ca. illicicola (LT DN 2.1 & LT DN2.2) .................. 29

4.6. Biological characteristics of fungi................................................................................. 29

4.6.1. Growth of the mycelium on different nutrient media .................................................. 29

4.6.2. Growth of the mycelium on PDA nutrient medium at different temperature scales:.... 32

4.6.3. Growth of the mycelium on PDA nutrient medium at different moisture scales.......... 33

5. DISCUSSION ................................................................................................................. 37

6. CONCLUSION ............................................................................................................... 39

REFERENCES