Quantitative determination of Piroxicam in pharmaceutical dosage using reversed-phase high performance liquid chromatographic method

Journal of Science and Technology, Vol. 39A, 2019

© 2019 Industrial University of Ho Chi Minh City

QUANTITATIVE DETERMINATION OF PIROXICAM IN

PHARMACEUTICAL DOSAGE USING REVERSED-PHASE HIGH

PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD

NGUYEN QUOC THANG

Chemical engineering faculty - Industrial University of Ho Chi Minh City,

[email protected],

Abstract. Piroxicam is a well known and very effective anti-inflammatory drug. Piroxicam can be

determined by various methods. Here, we introduced a reversed-phase high-performance liquid

chromatographic (RP-HPLC) method for the determination of piroxicam in tablets. A Hypersil BDS￾C18 column (4.0 mm x 125 mm), 5 μm particle size and Diode Array Detector at wavelength 334 nm

were used for separation and detection of piroxicam. The mobile phase was a mixture of MeOH and

0.03M potassium phosphate buffer in a ratio 80:20; v/v, set at a flow rate of 0.3 mL/min. Total

chromatographic analysis time per sample was approximately 7 min with piroxicam eluting with

retention times of 4.694 min. The linear range was collected from 20 to 80 mg/mL, with linear

regression S = 0.0878C - 0.0927, correlation coefficient R2

= 0.9994. The limit of detection (LOD) and

limit of quantification (LOQ) were 109 ng/mL and 363 ng/mL, respectively. The recovery was from

99.0% to 101.8%. This method is simple, precise and rapid for quantitative determination of piroxicam

in tablets.

Key words: Piroxicam, reversed-phase high-performance liquid chromatography, pharmaceutical

dosage.

1. INTRODUCTION

Piroxicam was (4-hydroxy-2-methyl-N-2-(pyridyl)-2H-1,2-benzothiazine-3-carboxamide 1,1-dioxide,

(Fig. 1). It was a well known and very effective antiinflammatory drug [1]. Piroxicam is a

cyclooxygenase inhibiting, non-steroidal anti-inflammatory drug (NSAID) which exhibited anti￾inflammatory, analgesic, and antipyretic activities in animal models [2]. It was used for musculoskeletal

disorders, dysmenorrhea, and postoperative pain and its long half-life enables it to be administered once

daily.

Figure 1: Chemical structures of piroxicam.

Piroxicam has been examined by various methods: spectrophotometric procedures,

spectrofluorometric methods, HPLC method and FT-IR spectrometry. A spectrophotometric method for

the determination of piroxicam in drug is based on the reaction of piroxicam and alizarin (I) or alizarin

red S (II) or alizarin yellow G (III) or quinalizarin (IV) which can be measured at the optimum

wavelength [3]. Another to determination of piroxicam based on the reduction of ferric ferricyanide into

ferro-ferricyanide (Prussian Blue), with maximum of absorbance at 760 nm [4]. Piroxicam can also be

determined by spectrofluorometric methods, the method is based on the oxidation of piroxicam with