Phân bố các gen mã hóa Coagulase ở các genotype của staphylococcus Aureus

TAP CHl NGHliN CO'U Y HQC

PHAN B6 CAC GEN MA HOA COAGULASE 6^ CAC GENOTYPE

CLIA STAPHYLOCOCCUS AUREUS

Le Khdnh Trdm\ Dlnh HIPU Dung^

Phgm Thj Thiiy Duang*, Nguyin VQ Trung*

^Bdnh vidn Bach Mai. 'Tmdng Dpi hpc Y Hd Npi

Staphylococcus aureus Id cdn nguydn cua nhiiu lopi nhiim trung khde nhau, tmng dd cd nhung bdnh rit

npng. Xdc djnh cdc gen quy dinh yiu td ddc luc ed y nghia quan trpng tmng chin dodn, nghien cuv vi ca

chi gay bpnh, djch ti hpc phdn tw. Nghidn cdu nhim xdc djnh swphdn bd cde gen md hda eoagulase d cdc

genotype ciia S. aureus phdn ldp tw nhdn vidn Idm vide tai ea sd dich vu dn udng d Hd Ndi- Kit qud cho thiy

sdu genotype cua gen ma hda eoagulase dd dwac xdc dfnh. cd 1 genotype chiim wu thi (66.2%). cdc

genotype phdn bd vdi ty Id khde nhau d cdc chdng phan ldp dwac tw mui vd tay. cd 91.2% ngwdi mang S.

aureus vdi 1 lopi genotype. 8.8% ngwdi mang S. aureus vdi 2 hodc 3 loai genotype. Sdu genotype cua gen

ma hda eoagulase da dwac xdc djnh. genotype 6 chiim wu thi (66.2%). Cd 91.2% ngwdi mang S. aureus

vdi 1 lopi genotype. Mdt ngwdi cd thi mang S. aureus vdn han 1 lopi genotype.

Tip khda: S. aureus, genotype, gen md hda eoagulase

I. DAT VAN D ^

Staphylococcus aureus la mOt trong nh&ng

vi khuln hay gdp nhit tren ca thl ngudi.

S.aureus cung la can nguyen eiia nhilu loai

nhilm triing khae nhau, trong dd thudng gap

la nhiem trung ngodi da, ngO dOc Uigc phim,

viem xuang tuy, nhilm khuln huylt, nhiem

trimg bOnh viOn [2]. Vj tri cu tru hay gdp nhit

ciia S.aureus la Id mui trudc. S.aureus ed thl

cu trii thudng xuyen. khdng thudng xuyOn va

khOng cu trii trOn ea thl ngudi [1]. Coagulase

Id mOt trong nh&ng ylu td dOc lgc ngogi bao

ciia S.aureus. Day Id enzyme lam dOng huylt

tuang vd la mOt chl dilm d l phdn biet

S. aureus vdi eae tu clu gdy benh khae [9].

Hien nay. eac nghien cuu v l dieh te hoc phan

tu ciia S. aureus cdn rat han chl. Gen ma hda

eoagulase cd tinh da hinh thdi. Viee phdn loai

dga vao gen md hda coagulase dan gian, cd

do nhay vd do dge hieu eao [5]. VI vdy, chimg

tdi filn hdnh d l tai nghiOn c&u nay vdi muc

tieu: Xdc djnh sg phan bd eac gen md hda

eoagulase d ede genotype eiia S.aureus phdn

ldp tu nhdn viOn lam vlOe tai ca sd djch vu dn

udng d Ha NOI.

II. 06l TU'aNG VA PHU'QNG PHAP

1. Ddi tugng

47 chiing S. aureus phdn ldp dugc tu tay

vd mui cua 103 nhdn viOn ldm viOc tgi 5 ca sd

djch vu dn udng d Ha NOi, tilp xiic trge filp

vdi th&c dn. Thdi gian nghien c&u: t& thdng

3/2009 din .9/2010.

2. Phuang phap

Lly benh phim bing tdm bOng vO triing

d ea 2 bdn tay vd 2 Id mui ngodi 4 lln, cdch

nhau 1 tuin. Nudi cly phdn ldp, luu gi& chiing

[1]. Tdch DNA. Tiln hdnh ky thudt PCR [6],

elt bdng enzyme Alul (hdng Fermentas,

Canada), mdy PCR (Mastercycler gradient,

Eppdorf, D&c), hO thdng diOn di (Bp diOn di

ngang Horizon®58-Gibeo-BRL, Anh), hO thdng

chgp anh (mdy Geldoe, hang BioRad, Phdp).

4 logi mdi:

C0AG1:5'-ATACTCAACCGACGACACCG-3'

TCNCYH 78 (1) - 2012