Nghiên cứu hình thái học ung thư biểu mô tế bào thận

TCNCYH 66(1)-201€

function, and 'stem cell' status of muscle satellite cells into cardiomyocyte - like cells. Biochemical

cells. Dev. Biol, 1 15 - 124. and Biophysical Reasarch Commucatidns 324,

5. Sell, S. (1990). Is there a liver stem cell? 481 -488 .

Cancer Res. 50, 3811 - 3815. 7. Zuk PA. (2002). Human adipose tissue is a

6. Winston S. N. Shim. (2004). Ex vivo source of multipotent stem cells.Moi Biol Cell 13:

differentiation of human adult bone marrow stem 4279 - 4295.

Summary

ISOLATION AND CULTURE MESENCHYMAI STEM CELLS FROM HUMAN BONE MARROW

Mesenchymal stem cells (MSCs) - the one of aldult stem cells are multipotent, and are capable of

differentiation into cell lineages that derive from many other tissues. Objective: to apply methods for

isolation and cutlure mesenchymal stem cells from human bone marrow for long time. Method: patient's

bone marrow without hematopoietic diseases (n = 5). Isolate and culture long time MSCs from human bone

marrow. Define surface markers df hematepdietic stem cells (CD34) and lymphdcytes (CD45). Result:

Successfuly isolated and cultivated five MSC samples from human bone marrow. Almost cultured cells had

the lack expression of CD34 and CD45. Conclusion: applied successfuly methods to isolate and culture

MSCs from human bdne marrew fdr long time.

Keywords: Mesenchymal Stem Cells, Cell Culture

NGHIEN CLfu HINH THAI HOC UNG THU" BIEU MO TE BAO THAN

NguySn Van HUng

• ' . Bg mdn Giii phiu benh - Trtfdng Dai hgc Y Hi Ngi

Ung thu bleu md te bio thin la benh hiem gap d Viet Nam. Muc tieu: nghiSn cifu hinh thii hgc ung thu

bieu md te bio thin theo phin loai ciia Td chdc Y ti Thi gidi nam 2004. Ddi tugng va pbuang phap nghien

cdu: 65 ca ung thu biiu md ti bio than duge nghien cdu vi hinh thii va duge ddi chieu giifa dai thi. vi vi

the. Kit qua: 65 trUdng hgp ung thU biiu md ti bio than duge dinh gii vi hinh thii hgc, gom 83,2%, (54 ca)

typ ti bio sing; 9,2%o (6 ca) typ nhii; 4,6%o (3 ca) ky mau; 1,5%o (1 ca) typ tuy vi 1,5%o (1 ca) typ khdng djnh

loai vdi kich thudc trung binh 6,44 - 3,34 cm (sap xep td 2 cm tdi 15 cm) va 84,6%o u cd ranh gidi rd. Ve ddi

ehiSu gida dai thi vi vi thi, ung thu blSu md te bao thin ti bio sing thudng ed gidi ban rd, md u die vi bd

(96%), maf ca'f u miu ving (84,6%o) vi cd thi tao it nang nhd (p < 0,05); tuy nhiSn, d mdt so typ md hgc

khic khdng cd su khic biet. Kit luan: do md hgc HSn quan vdi kich thudc u (p < 0,05). Chi typ ti bao sing

cd ton thuang die trUng ve dai thi

Tii khoa: ung thfl bieu mo te bao than, typ te bao sang, typ nhu, typ ky mau, typ tuy, typ khong djnh loai

I. DA T VA N D E theo vj tri dja ly va gidi. Nam gidi ml c benh cao

Ung thfl bieu md te bao than la u ac tinh gl'p 2 lan nfl gidi. Benh phd bien d Bae Au, Ble

nguyen phlt cua nhu md than, chiem 2 - 3% cac My, trong khi rat tha'p d chiu A, d e vflng Nam va

ung thfl ndi chung. Tan sd ml c benh khac nhau Trung My [9].

41