HPLC analysis of some bacteriostats in deodorant

J. Soc. Cosmet. Chem., 32, 163-173 (May/June 1981)

HPLC analysis of some bacteriostats indeodorant

sticks and soaps

RAJA G. ACHARI and DAVID CHIN, Research &Development

Center, Bristol- Myers Products Division, 225 Long Avenue, Hillside,

NJ 07207.

Received February 5, 1981.

Synopsis

A stability-indicating HPLC method for the determination of some bacteriostatsuch as Triclosan

(2,4,4'-trichloro-2'-hydroxydiphenylether) and TCC (3,4,4'-trichlorocarbanilide) is described. The liquid

chromatographic separation iscarried out using a •Bondapak Alkylphenyl column and the mobile phase

consisting of 1:1 (V/V) acetonitrile:water. The method had been documented to be precise and accurate

and has been successfully applied in assaying commercially available deodorant sticks and soap samples.

INTRODUCTION

Triclosan (I) (Irgasan DP-300 ©, 2,4,4'-trichloro-2'-hydroxydiphenylether) (Ciba-Geigy,

Greensboro, NC) is a commonly used bacteriostat indeodorant sticks and soaps. TCC

(II) (Monsanto, St. Louis, MO) (3,4,4'-trichlorocarbanilide)s mainly used in deodorant

soaps. Various methods have been reported for the analysis of these bacteriostats in

deodorants, but all of these methods suffer from certain drawbacks. Perfumes, other

UV absorbing substances, orchemical breakdown products of the bacteriostats often

interfere with ultraviolet and colorimetric methods (1,2). The gas chromatographic

procedure of Demars and Yates (3) for the analysis of TCC is cumbersome and

non-specific because the amines which are ultimately analyzed are also the probable

chemical degradation products of TCC. One of the two reported HPLC procedures

requires agradient elution system (4) and the second requires aradial compression

separation system (5). Both of the above systems are neither suitable nor available in all

laboratories for routine analysis; moreover, neither of the above HPLC methods has

been tested to determine whether it is "stability-indicating" due to the chemical

degradation of the bacteriostats inthe finished products.

The present study is aimed at developing astability-indicating simple isocratic HPLC

method for assaying triclosan in deodorant sticks and TCC in deodorant soaps.

Chromatographic parameters have been provided to assay both triclosan and TCC

should these be present in combination. The chemical structures of triclosan and TCC

are shown below.

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