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Hợp chất Stilbenoit kháng tế bào ung thư, hai axit P-anisic và Benzoic từ cây bách bộ lá nhỏ Stemona peirrei ở Lào
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Nguyễn Thị Nhàn và Đtg Tạp chí KHOA HỌC & CÔNG NGHỆ 102(02): 151 - 154
151
Ngày nhận bài:30/1/2013, ngày phản biện: 18/2/2013, ngày duyệt đăng:26/3/2013
A CYTOTOXIC STILBENOID AND TWO BENZOIC ACIDS FROM
STEMONA PEIRREI PLANTS GROWING IN LAOS
Nguyen Thi Nhan1
, Vong Anatha Khamko1,2
,
Nguyen Thu Ha1,3, Dang Ngoc Quang1
, Pham Huu Dien1*
1Hanoi National University of Education, 136 Xuan Thuy Str., Hanoi, Vietnam
2
Savannakhet University, K. Phomvihane Distr., Savannakhet Province, Laos
3Hanoi College of Pharmacy, Minhkhai Comm., Tuliem Distr., Hanoi, Vietnam
SUMMARY
Three non-alkaloidal metabolites, stemanthrene C, p-anisic and benzoic acids, were isolated from
the roots of Stemona peirrei plants, collected in Savannakhet province (Laos). Their structures
were elucidated by spectroscopic analyses. Stemanthrene C exhibited medium cytotoxic activity
against KB cell line with IC50 value of 82.03 µg mL-1
.
Keywords: Stemona peirrei, stemanthrene C, p-anisic acid, benzoic acid, cytotoxic activity
INTRODUCTION*
Various species of the genus Stemona
(Stemonaceae) are widely used in Vietnam,
Laos and other countries of southeast Asia as
an anticough remedy and for their
antiparasitic properties. Especially, extracts
from the tuberous roots of S.tuberosa Lour.,
S. saxorum Gagnep., S. cochinchinensis ...
have been recommended in Vietnamese,
Laos’ traditional medicine for a broad range
of applications, such as to relieve cough and
asthma, to be used for against enteric
helminths and ectoparasites on human and
cattle [1-4]. Chemical investigations on
Stemona genus have resulted in the isolation
of more than 90 different alkaloids, most of
which share the common pyrrolo[1,2-
a]azepine basic nucleus, a minor fraction also
containing the pyrido[1,2-a]azepine skeleton
[5-7]. However, the non-alkaloids
*
Tel: 0912 109836, Email: [email protected]
components of this genus have been rarely
investigated yet: less than 20 compounds,
mostly belonging to dihydrophenanthrenes
and dihydrostibenes [8,9]. Herein, we
describe the isolation and structural
elucidation of three non-alkaloidal
metabolites of Stemona peirrei, growing in
Savannakhet province (Laos).
EXPERIMENTAL
General
IR spectra were recorded on Shimadzu-FTIR
8101M spectrophotometer, using KBr disk,
NMR (1H, 13C NMR, DEPT, HSQC, HMBC)
spectra were recorded on a Brucker Avance
500MHz. The chemical shift (δ) values are
given in ppm with TMS as internal standard,
coupling constant J : by Hz). EIMS spectra
were recorded on a HP 5989B mass
spectrometer. LC-MS spectra were recorded
on an Agilent LC mass spectrometer. Silica
gel (Merck Co., Germany) was used for flash
chromatography. TLC was carried out on
precoated Si gel GF254 (Merck Co., Germany)