Hợp chất Stilbenoit kháng tế bào ung thư, hai axit P-anisic và Benzoic từ cây bách bộ lá nhỏ Stemona peirrei ở Lào

Nguyễn Thị Nhàn và Đtg Tạp chí KHOA HỌC & CÔNG NGHỆ 102(02): 151 - 154

151

Ngày nhận bài:30/1/2013, ngày phản biện: 18/2/2013, ngày duyệt đăng:26/3/2013

A CYTOTOXIC STILBENOID AND TWO BENZOIC ACIDS FROM

STEMONA PEIRREI PLANTS GROWING IN LAOS

Nguyen Thi Nhan1

, Vong Anatha Khamko1,2

,

Nguyen Thu Ha1,3, Dang Ngoc Quang1

, Pham Huu Dien1*

1Hanoi National University of Education, 136 Xuan Thuy Str., Hanoi, Vietnam

2

Savannakhet University, K. Phomvihane Distr., Savannakhet Province, Laos

3Hanoi College of Pharmacy, Minhkhai Comm., Tuliem Distr., Hanoi, Vietnam

SUMMARY

Three non-alkaloidal metabolites, stemanthrene C, p-anisic and benzoic acids, were isolated from

the roots of Stemona peirrei plants, collected in Savannakhet province (Laos). Their structures

were elucidated by spectroscopic analyses. Stemanthrene C exhibited medium cytotoxic activity

against KB cell line with IC50 value of 82.03 µg mL-1

.

Keywords: Stemona peirrei, stemanthrene C, p-anisic acid, benzoic acid, cytotoxic activity

INTRODUCTION*

Various species of the genus Stemona

(Stemonaceae) are widely used in Vietnam,

Laos and other countries of southeast Asia as

an anticough remedy and for their

antiparasitic properties. Especially, extracts

from the tuberous roots of S.tuberosa Lour.,

S. saxorum Gagnep., S. cochinchinensis ...

have been recommended in Vietnamese,

Laos’ traditional medicine for a broad range

of applications, such as to relieve cough and

asthma, to be used for against enteric

helminths and ectoparasites on human and

cattle [1-4]. Chemical investigations on

Stemona genus have resulted in the isolation

of more than 90 different alkaloids, most of

which share the common pyrrolo[1,2-

a]azepine basic nucleus, a minor fraction also

containing the pyrido[1,2-a]azepine skeleton

[5-7]. However, the non-alkaloids

*

Tel: 0912 109836, Email: [email protected]

components of this genus have been rarely

investigated yet: less than 20 compounds,

mostly belonging to dihydrophenanthrenes

and dihydrostibenes [8,9]. Herein, we

describe the isolation and structural

elucidation of three non-alkaloidal

metabolites of Stemona peirrei, growing in

Savannakhet province (Laos).

EXPERIMENTAL

General

IR spectra were recorded on Shimadzu-FTIR

8101M spectrophotometer, using KBr disk,

NMR (1H, 13C NMR, DEPT, HSQC, HMBC)

spectra were recorded on a Brucker Avance

500MHz. The chemical shift (δ) values are

given in ppm with TMS as internal standard,

coupling constant J : by Hz). EIMS spectra

were recorded on a HP 5989B mass

spectrometer. LC-MS spectra were recorded

on an Agilent LC mass spectrometer. Silica

gel (Merck Co., Germany) was used for flash

chromatography. TLC was carried out on

precoated Si gel GF254 (Merck Co., Germany)