Hội chứng Down và hội chứng Klinefelter trong cùng một cơ thể

TCNCYH Phu trtrdng 62 (3) - 2009

4. Sun C. C , Cheng C. Y., Chien C. S., et al in ocular surface maintenance and repair". Acta

(2005), "Role of Matrix Metalloproteinase - 9 in Ophthalmologyca, 192, pp. 91 -94 .

Ex Vivo Expansion of Human Limbal Epithelial ^ -^^^j ^ ^ p^ ^j^ ^ ^ ^^ ^-^^^ j_ ^ et al

Cells Cultured on Human Amniotic Membrane", (JOQO), "Reconstructidh of damaged corneas by

lOVS, 46(3), pp. 809-814 . transplantation of autologous limbal epithelial

5. Thoft R. A. (1989), "The role of the limbus cells", N Engl J Med. 343, pp. 86 - 93.

Summary

EVALUATING METHODS FOR PROCESSING OF RABBIT LIMBAL EPITHELIAL

EXPLANT FOR THE CULTURE OF THE LIMBAL EPITHELIAL SHEET

Processing of limbal epithelial samples is important to create limbal epithelial sheets. Objective: to

evaluate the efficiency of the three different ways to process limbal epithelial samples using dispase,

trypsin or nothing. Methods: cohort, descriptive study. Results: morphological evidence showed that in

group with enzymatic treatment by dispase, limbal epithelial stem cells were developed and expanded

more quickly than other groups. After 14 days of culture, the corneal epithelial sheets had 2 cell layers,

adhered permanently to the amniotic membrane substrate. The phenotype and structure of the cells after

culture like those of the normal epithelial cell most. Conclusion: enzymatic treatment of explants by

dispase gives the best result in explants culture.

Keywords: corneal limbal epithelium, limbal stem cells

HOI CHU'NG DOWN VA HOi CHU'NG KLINEFELTER

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TRONG CUNG MOT CO THE

Nguyen Van Rflc

Tntdng Dal hoc Y Eta Ngi

Lech boi kep cd nghia li su tdn tai hai nhidm sic the (NST) bat thitdng trong cimg mat ea the, li mgt

hien tugng tuang ddi hiem. Da sd nhitng trtfdng hgp lech bdi kep duge phit hien trong say thai Hen tiep.

Muc tieu: phit hien nhdng bat thtfdng ve NST ciia mgt tre cd bieu hien hgi ehifng Down va bd me. Boi

tugng va phuang phap nghien cdu: mgt be trai hai tudi cd da'u hieu dien hinh ciia hgi chdng Down. Benh

nhan li con thd nha't ciia cap vg chong hoin toin khoe manh, khi sinh ngUdi me 26 tudi, ngudl bd 27 tudi.

Nghien cifu NST td te bia bach du lympho miu ngoai vi da duge thuc hien d benh nhin vi bd me. Ket

quii: phim tich NST da phit hien d Ijenh nhan cd 48 NST, thifa 1 NST X trong cap gidi tinh XY (XXY - hdi

ehifng Klinefelter) vi tbda 1 NST 21 trang cap NST sd 21 (tnsomy 21 - hgi ehifng Dawn). Bd me cd karyotyp

li binh thudng. Ket luan: karyotyp ciia benh nhin la: 48,XXY,+21, karyotyp etia bd me la: 46,XX va 46,XY.

Tfl khoa: Trisomy kep, hgi chung Down, hoi chflng Klinefelter