Hạn sử dụng và hàm lượng Fe, Cu trong vitamin C viên sản xuất trong nước

Nghien CLFU - Ky thuat

- Diiu kien phan tich HPLC: he thdng HPLC,

Detector UV: 230nm, tdc dp ddng: 1 ml/phut, thi

tich tiem: 20^1, nhiet dp phdng thi nghiem, pha

dpng: Nude - methanol - acetonitril (45:50:5).

Phuang phap thu dp hoa tan ma chiing toi xay

dung CO su tuang quan tuyin tinh chat che giua

dien tieh peak va ndng dp andrographolid trong

khoang ndng dp khao sat (9,70 - 48,5 pg/ml) vdi

R^ ~ 1, dp lap lai (1,94%) va dp dung (102,86 -

104,79%) eiia phuang phap nim trong gidi han

eho phep eiia Duge Diin My 31, thdi gian phan

tich eho tung miu thieh hgp (~ 6 phiit).

Summar y

As Andrographolide, the main active

component of the Antinres capsule, is of low

water-dissolubility, for quality estimation of the

product a new assay method for the dissolubility of

Andrographolide from the capsule was studied.

The developed method showed good linear

relationship between the corresponding peak

areas and Andrographolide concentrations (Ff~1),

satisfactory repeatibility (RSD = 0.85%) and

accuracy (1026- 104.9%, RSD = 0.73%). These

all met the requirements by USP31. This offered

fundaments for building preparation formulas and

estimating the product quality, aftenA/ard.

Tai lieu tha m khao

1. Dugc diin Viet Nam 3, 2002.

2. British pharmacopoeia, 2008.

3. Pharmacopoeia of PRC, 2005.

4. 3f' US pharmacopoeia, 2008.

5. Nanthanit pholphana, Nucharnart

Rangkadilok et al. Determination and variation

of three active Diterpennoid in Andrographis

panieulata (Burm.f.) Nees. Phytochemical

analysis {2004), 15, 365-371.

6. The Merk Index, 13* edition, pi 06-107.

Han su* dung va ham lirang Fe, Cu trong r

vitamin C vien san xuat trong utfac

D a t va n de

Hien nay tren thj trudng Dugc phim Viet

Nam, eae ehi pham vitamin C rat phong phii vd

da dang, Han sii' dung ede ehi phim ndy dugc

ghi la 2 nam. Mpt vin d i rit ddng dugc quan

tam ddi vdi eae nhd san xuit vd kinh doanh

vitamin C la su bao dam duge ham lugng acid

L-ascorbie trong chi pham vitamin C.

Nhiiu cong trinh nghien euu da eho thiy, vdi

vai trd la xiie tae eho ede qud trinh oxy hda khir,

ham lugng cua cae kim loai Fe vd Cu trong ehi

phim vitamin C se Id mpt trong nhung yiu td ed

anh hudng rit ldn din chit lugng eua ehi phim.

Trong eong trinh nghien euu ndy, ehiing toi tiin

hanh khao sat hdm lugng Fe va Cu trong mpt sd

chi phim vitamin C dang luu hdnh tren thj trudng

vd blng cam quan, ddnh gid ehit lugng cdc ehe

phim vitamin C. Dua tren nhung kit qua thu

duge, dua ra mpt sd nhan xet vd khuyin eao v i

thdi ban su dung cua cae ehi phim ndy.

TAP CHI DUQC HQC - 8/2010 (SO 412 NAM 50)

Le Kieu Nhi, Phan Tuy, Pham Thi HSng Nga

Trudng dai hoc Dugc Ha Noi

Nguyen lieu va Phu'O'ng phap

Ddi tu'ang nghien CIFU

Cac miu vitamin C dugc lay ngau nhi§n tai

mpt sd hieu thudc d Hd Npi, vdi dang bdo chi

vd xult xu da dang, duge ky hieu lin lugt Id

VitCi, VitC2, VitCs... Vit Cg. Chung loai miu

duge liy la vien nen, vien nang cirng. Cdc san

phim diu 6uge san xuit trong nude tai cdc

Cong ty ed phin duge phim Trung uang vd dja

phuang, ngdy san xult va ban su dung eung

gin nhau.

Phu'ang phap nghien cu'u

Theo doi sw thay doi mau sac cua cac che

pham vitamin C

Chiing ta diu biit, vitamin C, khi bj phdn

hiiy, cho mdu vang. Vi vdy, de sa bp danh gia

ehit lugng che phim, chung tdi so sdnh mdu

sle cdc chi phim vitamin C tai thdi diem

nghien cuu va theo doi, so sdnh su thay ddi

mdu sle cdc ehi pham theo thdi gian vai diiu

23