Development_of_fermented_coconut_milk_(Cocogurt)_as_the_potential_of_functional_probiotic_product_ri

S140 Abstracts / Journal of Bioscience and Bioengineering 108 (2009)

S135–S146

FE-P2

Structural features and immuno-stimulating effect

of the polysaccharide fractions from Cordyceps

militaris

Mina Masuda, Akihiko Sakurai, and Mikio Sakakibara

University of Fukui, Fukui, Japan

Cordyceps militaris, which belongs to entomopathogenic

fungi, has been used for medicinal purpose due to its

physiological activities (1, 2), and its polysaccharide is one

of the attractive bioactive components. In this study, two

polysaccharide mixtures were obtained by the extraction of the

mycelium of C. militaris with hot water (crude endo￾polysaccharide) and the filtrate of the culture broth (crude exo￾polysaccharide). A fractionation by anion-exchange

chromatography with DEAE cellulose were performed, and the

mixtures were separated into 4 (Endo-Fr. I–IV) and 2 (Exo-Fr. I,

II) fractions. Among them, Endo- Fr. III, IV and Exo-Fr. II were

the bound fractions to DEAE cellulose in

10 mM Tris-HCl (pH 8.0). The immuno-stimulating activity of

each fraction was estimated by nitrite-producing levels of

RAW 264 macrophages. Endo-Fr. II, III, IV showed the

significant activity and that of Endo-Fr. IV was the highest.

The activity of the crude exo- polysaccharide mixture was

primarily attributed to the fraction eluted after Exo-Fr. II,

which contained trace level of polysaccharide. Endo-Fr. II and

III mainly consisted of mannose and galactose, and their

molecular weight were less than 100 kDa. On the other hand,

the major monosaccharide component of Endo-Fr. IV (the

protein content was higher than those of the other fractions)

was glucose and its molecular weight was 250 kDa. The

relation between immuno-stimulating activity and protein

content is under examination.

References

1. Ng, T. B. and Wang, H. X.: Pharmacological actions of Cordyceps, a

prized folk medicine, J. Pharm. Pharmacol., 57, 1509-1519 (2005).

2. Masuda, M., Urabe, E., Honda, H., Sakurai, A. and Sakakibara, M.:

Enhanced production of cordycepin by surface culture using the

medicinal mushroom Cordyceps militaris, Enzyme Microb. Technol., 40,

1199-1205 (2007).

doi: 10.1016/j.jbiosc.20 0 9.09.021

FE-P4

Development of fermented coconut milk (Cocogurt)

as the potential of functional probiotic product rich

in medium chain triglycerides

Tomi Ertanto, Tetuko Dito Widarso, RH Fitri Faradilla, Riyanti

Ekafitri, and Mujiono Mujiono

Bogor Agricultural University, Bogor, Indonesia

Indonesia has the largest coconut plantation area in the

world which has 3,8 million Ha and produces 3.3 million tons

equal as dry coconut (1). Coconut milk, as one of traditional

processing products of coconut potentially becomes main

ingredient for probiotics yoghurt making. As a source of

medium chain triglycerides, mainly composed by lauric acid

(C12), coconut milk has great potential as functional food. This

research was conducted to have the right Lactic Acid Bacteria

combination to make cocogurt. Viscosity, pH, and titrable

acidity were measured. Increasing of skim milk addition

was increasing pH of cocogurt and decreasing of titrable

acidity. Cocogurt viscosity was affected by lactic